缺血预处理对在体家兔房室结细胞形态结构的影响

Effect of ischemic preconditioning on the structure of atrioventricular node cells

目的观察缺血预处理在家兔急性心肌梗死模型中,对在体房室结细胞形态结构的影响。方法健康家兔30只,雌雄不拘,随机分为假手术组,缺血再灌注(IR)A组、B组,缺血预处理(IP)A组、B组,通过结扎右冠状动脉建立家兔房室结缺血再灌注模型,观察经过缺血预处理后,以不同的再灌注时间30min(A组)、180min(B组)为实验终点,检测心脏血心肌酶变化,HE染色和透视电镜观察房室结细胞的形态学变化。结果IP组较IR组心肌酶检测值显著降低,光镜下细胞空泡变性及细胞坏死IP组较IR组明显减轻。电镜下房室结细胞的线粒体、肌丝等超微结构在IP组损伤也明显减轻。结论缺血预处理对房室结细胞有保护作用。

Objective To evaluate the effects of ischemic preconditioning （IP） on the structure of atrioventricular node （AVN） cells in ischemia-reperfusion rabbit. Methods Thirty rabbits were divided into 5 groups in this experiment： normal group, IRA group, IRB group, IPA group and IPB group. IP and I/R models were establish by obstructing right coronary artery. Detect the change of serum AST, LDH, CK when the experiment were ended. The ultra microstructure of AVN cell were observed by HE staining and byperspective electron microscope. Results Myocardial ereatase and the rate of apoptosis was significantly higher in the IR group than in NC group （P〈0.05）,while it were significantly lower in the IP group than in the IR group （P〈0.05）.Damage of chondriosome and myofilament were ohviously minimal in IP than in IR group. Conclusion IR could induce AVN cells injury in rabbit,while ischemia preconditioning had a protective effect.