中药女贞子活性成分及其衍生物的薄层色谱分析

TLC studies on the active constituents of fructus ligustri lucidi and derivatives of the constituents

目的:为中药女贞子的品质评价及其活性成分的开发应用提供实验依据,以利于更全面地对药材进行质量控制和进一步开展活性单体的深入研究。方法:女贞子经乙醇提取后,对不同极性溶剂的萃取液和女贞子弱脂溶性有效成分红景天苷及其活性衍生物的薄层色谱行为进行考察和分析。结果:女贞子的最佳薄层色谱条件为:硅胶G板,氯仿和乙酸乙酯萃取液分别用环己烷-乙酸乙酯-乙酸(5∶2∶1)和乙酸乙酯-甲醇-甲酸(8∶1∶1)展开;红景天苷及其衍生物用乙酸乙酯-甲醇-乙酸(8∶1∶1)展开,10%硫酸乙醇液显色。结论:以上色谱条件可以满意分离和鉴别女贞子脂溶性和弱脂溶性活性成分及红景天苷衍生物。

Objective： To provide experiment data for the quality evaluation of fruetus ligustri lucidi and the application of its active constituents, in order to give comprehensive control of the quality of the crude drug and to study the active monomers deeply. Methods： TLC separation was studied on salidroside and its active derivatives with various solvents from ethanol extract of the crude drug. Results：Optimum TLC systems of fructus ligustri lucidi were： silica Gel（G）,cyclohexane-AcOEt- CH3 COOH（5 ： 2 ; 1） and AcOEt-MeOH-HCOOH（8 ：1 ： 1）for the chloroform extract and ethyl acetate extract, respectively, AcOEt-MeOH-CH3 COOH（8 ： 1 ： 1）for salidroside and its derivatives, 10 % H2 SO4 ethanol solution as developer. Conclusion; The TLC systems can be used to isolate and identify salidroside derivatives and the active constituents, which are either lipidsoluble or weak lipid soluble, from fructus ligustri lucidi.