大孔吸附树脂富集纯化牛膝总皂苷研究

Enriching and Purifying Achyranthes Bidentata Saponins with Macroporous Adsorptive Resins

目的应用大孔吸附树脂富集纯化牛膝总皂苷。方法牛膝醇提液上大孔吸附树脂柱,以水及不同浓度乙醇依次洗脱,考察牛膝总皂苷的洗脱率和纯度。结果牛膝总皂苷富集于50%乙醇洗脱液部分,洗脱率在58.42%以上。结论采用大孔吸附树脂方法富集、纯化牛膝总皂苷可行。

OBJECTIVE：To enrich and purify Achyranthes Bidentata Saponins with macroporous adsorptive resins.METHODS：The macroporous adsorptive resin columns on the Achyranthes Bidentata extractives were eluted respectively with water and different concentrations of ethanol,and the elution rate and purity coefficient of Achyranthes Bidentata Saponins were detected.RESULTS：The elution ratio of the portion of Achyranthes Bidentata Saponins that enriched in 50% alcohol eluant was above 58.42%. CONCLUSION：It is feasible to enrich and purify Achyranthes Bidentata Saponins with macroporous adsorptive resins.