摘要
选用定位于普通小麦7个部分同源群上的276对微卫星引物对普通小麦中国春和簇毛麦的基因组DNA进行扩增分析,有148对引物可在两个物种间检测到多态性。利用上述显示多态性的引物进一步对7个中国春-簇毛麦二体附加系进行扩增分析,筛选出分别可用来追踪簇毛麦1V至7V染色体的引物wmc49(1BS)、wmc25(2BS)、gdm36(3DS)、gdm145(4AL)、 wmc233(5DS)、wmc256(6AL)和gwm344(7BL)。此外还发现6DS上的微卫星引物gwm469可以用来追踪簇毛麦的2V染色体;2DS上的微卫星引物gdm107可用来追踪簇毛麦的6V染色体。进一步用涉及不同簇毛麦和小麦背景的小麦-簇毛麦染色体附加系、代换系和易位系进行扩增分析,这些微卫星标记也可用来鉴定簇毛麦的各条染色体。因此,这些簇毛麦染色体特异的微卫星标记可用来追踪普通小麦背景中的簇毛麦染色体。
Wheat （Triticum aestivum L.） microsatellite markers were screened for detecting Haynaldia villosa L. chromosomes introduced into wheat background. Two hundred and seventy six primer pairs mapped on 7 homeologous groups of wheat were used to amplify the gDNA of T. aestivum and H. villosa. The results showed that 148 of 276 microsatellite primers amplified polymorphic bands between common wheat cv, Chinese Spring and 11. villosa. Primers wine49 （1BS）, wmc25 （2BS）, gdm36 （3DS）, gdm 145 （4AL）, wmc233 （5DS）, wmc256 （6AL） and gwm344 （7BL） produced a specific polymorphic DNA fragment on chromosome IV to 7V ofH. villosa, respectively. In addition, gwm469 （6DS） detected a specific band on 2V; gdml07 （2DS） amplified a specific band on 6V. These microsatellite markers were effective in identifying individual H. villosa chromosomes in other T. aestivum-H, villosa chromosome addition, substitution and translocation lines involved in different 11. villosa accessions and wheat backgrounds. Therefore, these chromosome-specific microsatellite markers could be used as molecular markers for detection of chromosomes ofH. villosa in common wheat.
基金
This work was supported by the National High Technology Research and Development Program of China (863 Program) (No. 2001AA211051, 2003AA207100)Chinese National Natural Science Foundation (No. 30270827, 30471080)and Program for Changjiang Scholars and Research Team in University.
关键词
普通小麦
簇毛麦
小麦微卫星
Triticum aestivum
Haynaldia villosa
wheat microsatellites
