水稻穗瘟防卫反应相关基因的分离和鉴定(英文)

Isolation and Characterization of Defense Response Genes Involved in Neck Blast Resistance of Rice

以遗传背景相近、对叶瘟抗性相同但对穗瘟抗性不同的两个水稻株系为材料,利用抑制消减杂交(SSH) 技术构建穗瘟抗／感消减cDNA文库,经差异筛选及序列分析,共获得90个独立的差异表达cDNA克隆,根据与它们同源的基因功能推测,这些克隆可能参与了对病原菌的防卫反应、信号传导和转录等一些重要的生物学过程。利用RT-PCR分析了26个所筛选到的cDNA克隆在抗／感植株接种后的表达,17个基因的表达差异得到验证。对这些差异表达基因在抗感株系接种后不同时间点的表达谱也进行了RT-PCR的分析。文章首次报道了有关水稻对穗瘟抗性在mRNA水平进行研究,为深入研究水稻对穗瘟抗性的遗传机理打下了基础。

Two cDNA libraries enriched for transcripts ditlerentlally expressed in plants of two rice lines with similar genetic backgrounds and same leaf blast resistance but different responses to neck blast using suppression subtractive hybridization （SSH）. After differential screening and sequence analysis of the selected clones, 90 unique cDNA clones were found, of which 74 clones were with known functions according to the putative functions of their homologous genes in the database. They may be involved in pathogen response, signal transduction, transcription, etc. Expression differences of 17 out of the 26 selected cDNA clones in resistant and susceptible lines were confirmed by RT-PCR. Expression profilings of the 26 cDNA clones at the early stages after inoculation were also revealed by RT-PCR. This is the first report on the rice neck blast resistance at mRNA level and will facilitate the further study of genetic mechanism of neck blast resistance.