摘要
目的探讨应用高胰岛素—正葡萄糖钳夹技术(钳夹法)对模型动物进行胰岛素敏感性评价的可行性。方法雄性Wistar大鼠喂以富含不饱和脂肪酸的特殊高脂肪膳食5周。应用钳夹法评价大鼠的胰岛素敏感性。结果与常规喂养大鼠相比,高脂膳食组的葡萄糖输注率显著降低,空腹血浆胰岛素显著升高。同时,高脂膳食大鼠出现高脂血症。结论应用钳夹法评价大鼠的胰岛素敏感性较为灵敏、准确、可靠。
Objective To study the feasibility using euglycemic hyperinsulinemic clamp （EGHIC） technique. Methods Adult male Wistar rats were fed with high-fatty（59% of calories-fat） diet for 5 weeks. EGHIC technique was used to estimate their insulin sensitivity. Results Glucose infusion rates（GIR） in the rats with high-fat diet were lower than those in control rats. Meanwhile, hyperinsulinemia and hypertriglyceridemia were observed in the rats with high-fat diet. Conclusion EGHIC technique is sensitive,accurate and reliable in evaluating insulin sensibility in rats.
出处
《江苏医药》
CAS
CSCD
北大核心
2006年第3期238-240,共3页
Jiangsu Medical Journal
基金
重庆市自然科学基金资助(20038012)
