不同日龄下3品系果蝇的七氟醚ED_(50)和生存力

Influence of three strains of drosophila melanogaster's age on sevoflurance ED_(50) and vitality

[目的]了解3个果蝇品系在不同日龄下的七氟醚E队。变化趋势和生存力的差异。[方法]测定HYM3组、F．PS1组和F．PR1组三组果蝇的处女蝇在出生后1—8d、10、12、15、18、22、26、30、34、38、42d的七氟醚ED50，各组内每日龄上的七氟醚ED50均与本组第7日龄果蝇的七氟醚ED50进行t检验。3组雄蝇每组200只分5个培养管饲养，每3日更换1次培养基，并计数生存个体。按寿命表法计算各组果蝇的各项生存力数据。卡方检验比较各组的显著性差异性。[结果]在HYM3组、F．PS1组和F．PR1组中，与7日龄时比较，分别在26日龄以后、15日龄以后和22日龄以后出出了显著性差异和极显著性差异。其七氟醚ED50随日龄增加呈现出下降趋势。组内最小七氟醚ED50值与7日龄时的比值分别为0．70、0．65和0．70。组内变异系数较小的日龄段在HYM3组、F．PS1组和F．PR1组中分别为1～15日龄、1～10日龄和1～12日龄段。3组果蝇经寿命表法得出各项生存力指标无显著性差异。[结论]在3组果蝇品系中，各实验组的七氟醚ED50均随日龄的增长而有一定的降低，但3组改变的趋势基本一致，3组中七氟醚ED50的最小值与7日龄的七氟醚ED50值比值基本一致，分别为0．70、0．65和0．70。3个果蝇品系的各个生存力指标不存在显著性差异。

[ Objective] To assess the influence of the age of three strains of fruit fly on sevoflurance EDs0 and vitality. [Methods] According to the difference of age ranging from 1 to8, 10, 12, 15, 18, 22, 26, 30, 34, 38, and 42 days in turn, determine a series of flies＇ sevofluance EDs0 in three groups divided into HYM3, F. PS1 and F. PR1. Every result was given at test with 7 - day - old fly＇ s sevafluance ED50 in each group. 200 male fruit flies from each group were fed with 5 test tubes, and test cubes were renewed every 3 days, counting the number of survival. The data about vitality per group were computed by life table. The significance among these 3 groups was processed by ANOVA. [Results] Compared with the sevoflurance ED50 of 7 - day - old flies by test, the data showed significant difference after 26 days old in HYM3 group, 15 days old in F. PS1 group, and 22 days old in F. PR1 group. The sevoflurance ED50 decreased gradually as the age of flies increased. The ratios between the minimum ED50 and the 7 - day- old ED50 in HYM3, F. PS1 and F. PR1 groups were 0.70, 0.67, and 0.70. There was no significant difference in the vitality data of the three groups by life table. [ Conclusion] The results suggested that the vitality data had no significant difference in the three groups.