凋亡调控蛋白Bcl-2、Bax、P53和PDCD5在多囊卵巢综合征各级卵泡的表达

The expression of apoptosis-related protein Bcl-2、Bax、P53 and PDCD5 in granulosa cells of the PCOS

目的检测凋亡调控蛋白Bcl-2、Bax、P53和PDCD5在多囊卵巢综合征和对照组各级卵泡中颗粒细胞的表达,探讨颗粒细胞凋亡与PCOS的发病可能的相关关系。方法选择因多囊卵巢综合征行卵巢楔形切除的卵巢病理组织32例,同时选择年龄相匹配并于卵泡期手术的非多囊卵巢综合征卵巢组织15例为对照组,采用免疫组化法检测凋亡调控蛋白Bcl-2、Bax、P53和PDCD5在各级卵泡中颗粒细胞表达情况,由计算机提取,定量分析各级卵泡颗粒细胞免疫组化着色的光密度(OD)值,比较两组间的差异。结果Bcl-2、Bax、P53和PDCD5蛋白在多囊卵巢综合征始基卵泡、窦前卵泡颗粒细胞的表达与正常对照组比较差异无显著性(P>0.05)。窦状卵泡颗粒细胞Bcl-2蛋白表达下调,Bax和PDCD5的表达明显上调,与对照组比较其OD值差异有显著性(P<0.05)。P53蛋白在多囊卵巢综合征各期卵泡颗粒细胞的表达差异无显著性,与正常对照组比较差异无显著性。结论多囊卵巢综合征始基卵泡和窦前卵泡期颗粒细胞的凋亡与对照组比较差异无显著性,窦状卵泡期颗粒细胞的凋亡明显增加。Bcl-2、Bax和PDCD5细胞浆表达凋亡调控蛋白参与了卵巢颗粒细胞的凋亡过程,P53细胞核表达的凋亡调控蛋白在多囊卵巢综合征颗粒细胞的凋亡过程中与对照组比较无明显差异。

Objective To investigate the expressions of Bel-2, Bax, P53 and PDCD5 in granclosa cells of polycystic ovary syndrome （PCOS）, and to explore the pathogenesis of PCOS. Methods The tissues from 32 cases of PCOS and 15 cases of healthy control were examined immunohistochemically. The expression of Bcl-2, Bax, P53 and PDCD5 preteins were measured by optical density （OD） with microscope and analyzed by computer imaging technique software. The results were analyzed with independent-samples T test. Results The expressions of Bcl-2, Bax, P53 and PDCD5 proteins on granulosa cells presented no difference in primordial follicle and preantral follicle of PCOS, but significant downregalation of Bcl-2 and upregulation of Bax, PDCD5 in antral follicle granulosa cells of PCOS compared to normal control group were observed. No significant difference of P53 expressions was found between follicle granulosa cells of PCOS and the normal control group. Conclusions The apoptosis of granulosa cells in primordial follicle and preantral follicle of PCOS is not different from the control group. The apoptosis of granulosa cells is more significant in antral follicle of PCOS than the control group. The expressions of Bcl-2, Bax, and PDCD5 in cytoplasm are important apoptotic factors in the granulosa cells of ovary; and the expression of P53 in nucleus has no correlation to the granulosa cells aoootosis in this study.