银屑病患者外周血单一核细胞的培养上清液对骨髓造血干细胞和祖细胞集落形成的影响

The influence of culture supernatant of psoriatic PBMCs on the colony forming of bone marrow-derived hematopoietic stem cells and progenitor cells

目的研究银屑病患者外周血单一核细胞(PBMC)的培养上清液对骨髓造血干细胞、祖细胞集落形成的影响。方法密度梯度离心法分离骨髓单一核细胞,采用甲基纤维素半固体集落培养法研究银屑病患者及正常人PBMC培养上清液对骨髓高增殖潜能集落形成细胞、红系集落形成单位及粒细胞-巨噬细胞系集落形成单位集落形成的影响。结果银屑病患者PBMC培养上清液作用后,骨髓高增殖潜能集落形成细胞、红系集落形成单位及巨噬细胞系集落形成单位集落形成数较自然增殖组及正常人PBMC培养上清液组显著减少(P<0.05,P<0.01);而正常人PBMC培养上清液作用组与自然增殖组差异无统计学意义(P>0.05)。结论银屑病患者PBMC培养上清液对骨髓造血干细胞、祖细胞集落形成可能有影响。

Objective To study the influence of culture supematant of psoriatic peripheral blood mononuclear cells （PBMCs） on the colony-forming of bone marrow-derived hematopoietic stem cells and progenitor cells. Methods Bone marrow-derived mononuclear cells were separated by density gradient centrifugation. Methylcellulose semi-solid culture medium was used to culture the bone marrow mononuclear cells in culture systems. The PBMC culture supernatant from psoriatic patients or normal controls were added to the culture, to observe their influence on the marrow-derived high proliferative potential colony forming cells （HPP-CFCs）, erythroid and granulocyte-macrophage colony forming units （CFUs-E and CFUs-GM） of bone marrow hematopoietic stem/progenitor cells from healthy individuals. Results The values of HPP-CFCs, CFUs-E and CFUs-GM were significantly lower in the bone marrow cells stimulated by the supematant of cultured psoriatic PBMCs than those stimulated by the supematant of cultured normal PBMCs and those in the spontaneous proliferation group （P 〈 0.05, P 〈 0.01）; however, no significant difference of those values was found between the group stimulated by supematant of cultured, normal PBMCs and the spontaneous proliferation group （P 〉 0.05）. Conclusions Psoriatic PBMCs have specific biological activity and can inhibit the colony forming of bone marrow hematopoietic cells from healthy individuals.