幽门螺杆菌阳性十二指肠球部溃疡患者红细胞免疫功能的变化

Immune function of erythrocyte in patients with helicobacter pylori positive duodenal ulcer

目的探讨幽门螺杆菌(Helicobecterpylori)阳性十二指肠球部溃疡患者红细胞免疫功能的变化。方法47例H.pylori阳性十二指肠球部溃疡患者和32例健康体检者入选,前者以标准方案治疗7天,后者不予处理作为对照。治疗前后分别以流式细胞仪测定其血液红细胞上CR1的数量,以免疫黏附酵母菌花环法测定其活性。结果与正常人相比,H.pylori阳性十二指肠球部溃疡患者红细胞C3b受体花环率、红细胞CR1数量降低(P<0.01),红细胞免疫复合物花环率增高(P<0.01);根除H.pylori治疗后,上述参数恢复到正常水平。结论H.pylori阳性十二指肠球部溃疡患者红细胞免疫功能低下,H.pylori的感染可能是导致其红细胞免疫功能低下的原因。

Objective To investigate the immune function of erythrocyte in the patients with Helicobacter pylori （ H. pylori）-positive duodenal ulcer. Methods Forty-seven patients with Hp-positive duodenal ulcer（DU） and 32 healthy individuals were enrolled. The patients was treated with standard therapy for 7 days. Before and after the treatment, the number of complement receptor type 1 （CR1） on eryth/ocytes was detected by flow cytometry and the CR1 activity was determined by yeast rosette method. Results Compared with control, the number of CR1 on erythrocytes was significantly decreased, （18.96 ± 4.79）% vs （24.57 ± 5.82）%, P 〈 0.01. The activity showed by the rosette rate of RBC-C3b receptor of CR1 was significantly lower in patient group and RBC immune complex. After 7 days of treatment, all of the above parameters in patients with DU were returned to normal level. Conclusion The immune function of erythrocytes is lower in patients with duodenal ulcer, which might result from H. pylori infection.