腰椎间盘损伤后MRI表现及组织学改变的实验研究

A study of histology and MRI in goat lumbar intervertebral disc after lesion

目的:探讨山羊腰椎间盘损伤后不同时间点MRI表现和组织学改变。方法:15只山羊,人为刺伤L5/6椎间盘前纤维环全层和L6/7后部纤维环内层,在伤后3周、3个月、6个月进行腰椎MRI检查观察髓核和纤维环的信号改变,光镜下观察实验椎间盘后部纤维环组织学改变。结果:L6/7椎间盘损伤后部纤维环内层后,在实验期间未发生愈合,通过MRI定性、定量分析发现,椎间盘出现退变,并随时间进行性加重(3周,3、6个月),且退变过程较L5/6加速。T2像上未发现一例出现HIZ。结论:经山羊椎间盘前方损伤后部纤维环内层后,纤维环内层很难愈合,早期出现椎间盘退变。

Objective： An in vivo study of histology and MRI of goat intervertebral disc after lesion in different time points.Method：The whole lamellae of the anterior annulus fibrosus of L5/6 intervertebral discs and the inner site of the posterior annulus fibrosus of L6/7 intervertebral discs of 15 Boer goats were stabbed,and lumbar MRI and histological test were performed after 3 weeks,3 months and 6 months in order to observe the signal changes of nucleus pulposus and anulus fibrosus.Result：Inner part of L6/7 posterior annulus fibrosus disruption did not healed during experimental period,the qualitative and quantitative MRI revealed progressive severe intervertebral disc degeneration over time （in 3 weeks,3,6 months） compared with L5/6 intervertebral disc.Of all experimemtal intervertebral discs,no HIZ could be observed in MRI.Conclusion：Mter stabbing the posterior inner part of annulus fibrosus by anterior approach,it＇s hard to heal,which can result in early intervertebral disc degeneration.