摘要
目的:探讨TIM P-3对内皮细胞增殖的抑制作用及特点。方法:采用形态学观察、M TT法、[3H]-TdR掺入法流式细胞仪分析等技术方法观测不同浓度TIM P-3作用下人脐静脉内皮细胞增殖活性的变化。结果:①不同浓度(10～100ng/m l)的TIM P-3处理H UVEC细胞不同时间(1～7天)后,生长均有显著抑制作用且呈现明显的剂量和时效依赖关系。②经TIM P-3处理后,H UVEC细胞增殖活性降低,主要表现为有丝分裂计数降低,细胞的生长抑制率及移行抑制率增加。③细胞生长减慢,TIM P-3能有效抑制G1、S期的细胞;抑制细胞从G2期进入M期;同时促进细胞的凋亡等。结论:TIM P-3具有抑制内皮细胞增殖、迁移、促进细胞凋亡,进而抑制血管生成的作用。
Objective： To investigate the inhibitory effect of TIMP-3 on endothelial cell （EC） proliferation. Methods： The inhibition of EC proliferation was analyzed by light microscopy, MTT, [^3H]-TdR up-take, and flow cytometry et al. Results： A concentration-dependent and time-dependent proliferation inhibition and survival were demonstrated in the HUVEC incubated for 1-7days in the presence of TIMP-3. The proliferation inhibition was shown as the lessened cell growth, decreased counting of mitotic cells. TIMP-3 could also aggravate the increase of apoptotic cells and cell ratio in G2/M phase. Conclusion： TIMP-3 has significant inhibitory influence on EC proliferation, migration and can promote apoptosis of cells.
出处
《重庆医科大学学报》
CAS
CSCD
2006年第1期26-30,34,共6页
Journal of Chongqing Medical University
基金
重庆市卫生局科研基金(渝卫科教[2001]-01-2-072)
