PEDF抑制大鼠角膜移植术后新生血管作用的实验研究

Experimental study on inhibitory effects of PEDF on rat cornealneovascularization after penetrating keratoplasty

目的:探讨局部应用色素上皮细胞衍生因子(pigm entepithelium derived factor,PEDF)滴眼液抑制角膜移植术后角膜新生血管(cornealneovascularization,CNV)的作用及其可能机制。方法:建立大鼠同种异体间穿透性角膜移植模型,术后把72只大鼠随机平均分为实验组(10nM的PEDF眼液)和对照组(赋形剂滴眼液)。对术眼角膜用裂隙灯显微镜观察和照相,定量分析角膜新生血管面积,术后1w,2w,3w,1月,2月,3月,每个组随机处死6只动物,取其角膜标本进行免疫组化染色和透射电镜观察。结果:实验组角膜新生血管面积较对照组在术后1w,2w,3w,1月,2月显著减少(P<0.01或P<0.05)。结论:局部应用PEDF滴眼液可以抑制角膜移植术后角膜新生血管生长。

Objectve： To evaluate the effects of pigment epithelium derived factor（PEDF） eyedrops in inhibiting corneal neovascularization （CNV） by topical installition on the rat model undergoing penetrating keratoplasty and to investigate its possible mechanism and its clinical significance. Methods： Penetrating karatoplasty were proformed orthotopically from Wistar rats to SD rat＇ recipients. 72 SD rats were randomly assigned to two groups-experimental group and control group, after transplantation. Experimental group was treated with PEDF eyedrops and control group was treated with escipient eyedrops. CNV was observed by the slitlamp biomicroscope examination. The area of CNV was caculated quantitatively. 6 animals in both groups were killed respectively at the 1st week, 2nd week, 3rd week, 1st month, 2nd month and 3rd month postoperatively. PEDF expression was examined by immunohistochemistry and corneal untrastructural morphology was observed by TEM. Results： The area of CNV was decreased at 1 week, 2 weeks, 3 weeks, 1 month and 2 months postaperatively （ P〈0.01 or P〈0.05 ）. Significantly increased PEDF expression was also observed in the experimental group by immunohistochemistry staining, as compared with the control group. Conclusion： Topical application of PEDF eye drop can prevent vascular growth of corneal allografts.