HSP90的抑制剂Geldanamycin对乳腺癌细胞侵袭、转移能力的影响

Effect of HSP90 inhibins geldanamycin on invasion and metastasis of human breast cancer cell line

目的:探讨H SP90抑制剂Geldanam ycin(GA)对高转移性乳腺癌细胞株M DA-MB-231、MDA-M B-435s侵袭、转移能力的影响。方法:噻唑兰比色(M TT)法检测两株细胞对人工基底膜(Matrigel)的粘附能力变化;TranswellCham ber体外侵袭运动实验研究GA处理前后M DA-M B-231、M DA-M B-435s细胞侵袭、运动能力的改变。结果:GA处理后的M DA-M B-231和M DA-MB-435s细胞的粘附、侵袭、运动能力与未处理组细胞相比均明显下降,差异具有显著性(P<0.05和P<0.01)。结论:H SP90抑制剂Geldanam ycin(GA)能够明显抑制乳腺癌细胞株M DA-MB-231、MDA-M B-435s粘附、侵袭和运动能力。

Objective： To investigate the effects of HSP90 inhibitors Geldanamycin （GA） on invasion and motility potential in human breast cancer cell. Methods： The effects of HSP90 inhibitor GA on the adhesion,invasion and metastasis of human breast cancer line MDA-MB-231 and MDA-MB-435s in vitro were explored by MTT colorimetric and transwell chamber. Results： Treatd with GA, the adhesion, invasion and motility abilities of the MDA-MB-231 and MDA-MB-435s cells were decreased significantly as compared with the control guoup, （P〈0.05 and P〈0.01）. Conclusion： The abilities of adhesion, invasion and motility of breast cancer cells can be suppressed by the inhibition of GA.