丝裂霉素C联合蛙皮素增强抗胃癌作用的体外实验

Reinforcement of anticancer effect of combined mitomycin and bombesin on human gastric cancer cell in vitro

目的:探讨丝裂霉素C联合蛙皮素在体外诱导人胃癌细胞的凋亡作用。方法:利用体外细胞培养、MTT比色技术及流式细胞仪等方法,在对照组、丝裂霉素C(MMC)组、蛙皮素(BOM)组和MMC+BOM组中,用不同浓度的BOM及MMC,对人胃癌细胞株SGC7901的生长曲线、细胞抑制率和细胞凋亡率的影响。结果:在一定浓度范围内,BOM对胃癌细胞的促生长作用及MMC的抑制作用均呈时间-剂量依赖性。BOM组、对照组、MMC组和MMC+BOM组的胃癌细胞生存率依次为90.00%、82.96%、65.07%、60.14%;凋亡率在MMC+BOM组明显增高(29.26%),与对照组(4.72%)或BOM组(5.13%)两两比较时,具有显著性差异(P<0.01);MMC+BOM组凋亡率(29.26%)较M M C组(25.11%)高,在统计学上具有差异性意义(P<0.05)。结论:虽然BOM在体内外能刺激胃癌细胞增殖,但与抗癌药MMC联合后,可协同MMC对胃癌细胞的凋亡,提高抗癌药的疗效。

Objective： To study the effects of combined mitomycin （ MMC ） and bombesin （ BOM ） on apoptosis of human gastric cancer cell in vitro. Methods： Four groups were formed： control group, MMC group, BOM group and MMC＋BOM group. The effects of different concentrations of MMC and BOM, on the rate of cell existence, and cell apoptosis in human gastric cancer line SGC 7901 were studied in four groups by using cell culture, MTT assay and flow cytometry. Results ： Within the scope of certain density, the multiplication effect of BOM, and the inhibitory action of MMC on stomach cancer cell line SGC 7901 were in a time and dose dependent manner. The rates of cell existence in four groups were respectively 90.00% in BOM group,82.96% in control group,65.07% in MMC group and 60.07% in MMC＋BOM group, lowered one by one in order. There was significant difference in statistics about the rate of apoptosis between MMC＋BOM group （ 29.26% ） and control group （ 4.72% ） or BOM group （ 5.13% ） （ P〈0.01 ）. The rates of apoptosis in MMC＋BOM group （ 29.26% ） and MMC group （ 25.11% ） had difference in statistics （ P〈0.05 ）. Conclusion： BOM cooperating with MMC could effectively induce the apoptosis of gastric cancer cells though it accelerates the multiplication of human gastric cancer cells in vivo or in vitro. So it could increase curative effects of anticancer agent （ MMC ） on cancer cells.