输注同种异基因凋亡淋巴细胞在受体内的分布

Distribution of allogeneic apoptotic spleen lymphocytes transfused into recipient mouse

目的探讨供体凋亡淋巴细胞诱导移植耐受模型中转输细胞的作用。方法用荧光标记供体脾淋巴细胞,诱导凋亡后分离纯化。将标记细胞经尾静脉输入受者,于不同时间点取肝、脾、肺脏进行荧光细胞分布观察与测定。结果除最初30 min内荧光标记细胞在肺脏短暂汇集,从开始到其后12 h内一直以肝脏内荧光细胞比例最高。脾脏内荧光细胞比例远远低于肝脏。12 h各器官荧光细胞基本消失。结论异源供者凋亡淋巴细胞经转输后主要聚集并驻留于肝脏。鉴于肝脏本身的特殊免疫功能,初步推测肝脏为转输异源凋亡细胞发生作用的关键位点。

Objective To analyze the fate of allogeneic apoptotic lymphocytes transfused into recipient mouse. Methods Allogeneic spleen lymphocytes stained with fluorescein were induced to apoptesis, and then transferred into recipients after purification. Livers, spleens, and lungs of the mice were analyzed by FACS and fluorometry microscopy for investigating the fate of the labeled cells at various time points. Results Except that some of the labeled apoptotic cells accumulated in lung in the first 30 min, from the beginning to 12 h, most of labeled cells accumulated in liver. Few labeled cells appeared in spleen. Twelve hours after transfusion, the labeled cells disappeared in all the three organs. Conclusion The labeled allogeneic apoptotic cells mainly trap into liver after transfusion. Considering the immunologic peculiarities of liver, the key process in allogeneic apoptotic cells-induced tolerance is speculated to take place just in the liver.