五种与癫癎治疗靶点相关蛋白质在颞叶癫癎模型海马组织中的表达

Novel therapeutic target proteins expressed in hippocampi of rats with temporal lobe epilepsy

目的寻找颞叶癫大鼠海马组织的差异表达蛋白质,为寻找新的癫治疗靶点和研发新的治疗手段打下基础。方法运用二维电泳和MALD I-TOF-MS技术,对比分析氯化锂-匹罗卡品致大鼠海马组织和正常大鼠海马组织的蛋白质表达谱,对发现的差异表达蛋白质进行分析和鉴定。结果在氯化锂-匹罗卡品致大鼠海马组织中筛选到32个差异表达蛋白质斑点,其中20个在癫组表达下调,12个在癫组表达上调,其中5个蛋白质已被最终鉴定确认,分别为:突触结合蛋白Ⅰ(synaptotagm inⅠ)、神经丝蛋白(neurofilam ents,NF)、热休克蛋白27(heat shock prote in 27,HSP27)、电压依赖性阴离子通道1(voltage-dependent an ion channel prote ins 1,VDAC1)和异柠檬酸脱氢酶(isoc itric dehydrogenase,ICD),其中突触结合蛋白Ⅰ可能为潜在的新治疗靶点。结论颞叶癫大鼠海马组织中存在大量差异表达蛋白质,部分可能为潜在的癫治疗靶点。

Objective To investigate the expression profile of proteins in rat hippocampus with temporal lobe epilepsy （TLE）, as to further explore the pathogenesis mechanism and the potential clinical therapeutic targets. Methods The expression profile of proteins of both rats with TLE induced by LiCl-PILO and normal rats was compared and analyzed by using 2-dimensional electrophoresis （2-D electrophoresis） and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS）. Results Thirty-two protein spots differentially displayed were screened out, in which 20 proteins were detected down regulated and 12 were up regulated and 5 proteins among them were finally identified. There are synaptotagmin Ⅰ, neurofilaments, heat shock protein 27, vohage-dependent anion channel proteins 1 and isocitric dehydrogenase. Conclusion There are many differentially expressed proteins in rat hippocampus with TLE and some of them might be potential clinical therapeutic targets.