三氧化二砷联合氟尿嘧啶体外对人类大肠癌细胞生长及端粒酶活性的影响

The influence of arsenic trioxide combined with fiuorouracil on the growth and telomerase activity of human large intestine carcinoma cells

目的:探讨三氧化二砷(As2O3)注射液与氟尿嘧啶(5-FU)联合应用对人类大肠癌细胞的生长及其端粒酶活性的影响。方法:采用四甲基偶氮唑蓝比色(MTT)法观察As2O3注射液和/或5-FU对人类大肠癌细胞株LoVo和CoLo-320生长的影响;应用端粒重复序列扩增法(TRAP)—聚丙烯酰胺凝胶电泳(PAGE)银染法检测As2O3注射液和/或5-FU处理前后大肠癌细胞端粒酶活性的变化。结果:与As2O3或5-FU单药作用相比,As2O3与5-FU联合应用可显著增强抑制人结肠癌细胞增殖作用,并对细胞端粒酶活性具有增强抑制作用。结论:与各单药相比较,As2O3注射液联合5-FU能够明显增强抗大肠癌作用,其机制可能与增强抑制癌细胞的端粒酶活性有关。

Objective：To study the influence of arsenic trioxide （As2O3 ） injection combined with/or fiuorouracil （5-FU） on the growth and telomerase activity of human large intestine carcinoma cell lines. Methods：The influence of growth on human large intestine carcinoma cell lines LoVo and CoLo-320 induced by As2O3 injection combined with/or 5-FU was analysed by 3-（4,5-dimethytthiazole-2-yl ）-2, 5-diphenyl tetrazolium bromide （MTT） assay. Telomerase activity of the carcinoma cells was measured by the method of Repeat Amplitcation Protocol （TRAP） combining with Polyacrylamide Gel Electrophosis （PAGE） Silver Staining method before and after treatment of As2O3 injection combined with/or 5-FU. Results：As203 injection combined with 5-FU may obviously improve the anti-tumor effeet on human large intestine carcinoma, and may strengthen inhibiting the tolemerase activity comparing each individual drug. Condusion：As2O3 injection combined with 5-FU may obviously improve the anti-tumor effect on human large intestine carcinoma, and whose mechanism maybe related to its strengthen inhibiting the tolemerase activity.