果寡糖和甘露寡糖对断奶仔猪免疫机能的影响

Effects of Fructo-oligosaccharides and Mannan Oligosaccharides on Immune Response in Weaned Pigs

研究探讨了果寡糖和甘露寡糖对断奶仔猪免疫机能的影响。实验采用随机区组设计法将48头健康、体重一致的35日龄断奶杜洛克仔猪分为4组,每组3次重复,每个重复4头进行为期28天的饲养实验。4个处理组分别为:基础日粮+0.5%果寡糖(FOS组)、基础日粮+0.3%甘露寡糖(MOS组)、基础日粮+0.45%果寡糖+0.25%甘露寡糖(F+M组)、基础日粮+75 mg/kg金霉素+40 mg/kg洛克沙生(ABT组)。实验结果表明:(1)寡糖组免疫器官指数较ABT组大(P>0.05)。(2)在断奶后第7天,FOS组(0.32±0.49)、MOS组(0.32±0.04)和F+M组(0.32±0.07)均较ABT组(0.20±0.01)显著提高了血清免疫球蛋白A(IgA)的浓度(P<0.05),同时FOS组(3.50±0.49)较ABT组(2.68±0.47)显著提高了IgG浓度(P<0.05);F+M组显著提高了断奶后第21天IgG浓度(P<0.05);MOS组显著提高了断奶后第21天IgM浓度(P<0.05)。(3)与ABT组相比,FOS显著提高了断奶后第7天胸腺(21.33±6.03对37.33±4.04)、脾脏(19.67±2.08对30.33±8.73)、淋巴结(24.67±4.16对37.67±2.52)CD4+T淋巴细胞百分数(P<0.05);FOS组(21.33±4.04对32.00±5.29)、MOS组(21.33±4.04对37.33±3.21)、F+M组(21.33±4.04对32.00±3.60)显著提高了断奶后第28天胸腺CD4+T淋巴细胞百分数(P<0.05);MOS组(20.67±3.51对29.67±5.51)显著提高了断奶后第7天胸腺CD8+T淋巴细胞百分数;F+M组(17.00±1.00对22.67±3.06)显著提高了断奶后第7天脾脏CD8+T淋巴细胞百分数(P<0.05);各寡糖组对断奶后第28天CD4+、CD8+T淋巴细胞百分数影响不显著(P>0.05);ABT组(34.33±5.03)在断奶后第7天较F+M组(20.67±6.43)显著提高了淋巴结CD8+T淋巴细胞百分数(P<0.05);在断奶后第7天,FOS组(1.42±0.32)、MOS组(1.52±0.46)、F+M组(1.51±0.30)淋巴结CD4+与CD8+比值显著(P<0.05)大于ABT组(0.71±0.03);在断奶后第28天,FOS组(1.36±0.21)和MOS组(1.34±0.16)脾脏CD4+与CD8+比值显著(P=0.01)大于ABT组(0.94±0.29),除此之外,各寡糖组CD4+与CD8+比值较寡糖组大(P>0.05),寡糖组间差异不显著。

The effects of fructo-oligosaccharides（FOS） and mannan-oligosaccharides （MOS） on immune response was studied in weaned pigs. Fourty-eight 35days old weaned Duroc x Landrace-Yorkshire pigs were divided into four groups with three replicates, each of which including 4 piglets, in a randomized block design. The four groups were fed with basic diet containing 0.5 % FOS（ group FOS）, containing 0.3 % MOS（ group MOS）, containing 0.45 % FOS and 0.25 % MOS（ group F ＋ M）, containing 75 mg/kg Chloreycline and 40 mg/kg Roxarsone（ group ABT） , respectively. The results showed that ： （ 1 ） The index of thymus, spleen, lymph node in group FOS, MOS, F ＋ M were higher than that in group ABT（P 〉 0.05）. （2）On the 7th day after weaning, the concentrations of immunoglobulin（IgA） in group FOS （0.32 ± 0.08）, MOS（0.32 ± 0.04）, F ＋ M （0.32± 0.07 ） significantly higher than that in group ABT （0.20 ± 0. 01 ） （ P 〈 0.05）, at the same time, the concentrations of IgG in group FOS（ 3.50 ± 0.49 ） were higher than that in group ABT（2.68± 0.47 ） （ P 〈 0.05 ） ; Compared with antibiotics, F ＋ M increased the concentration of IgG markedly （P 〈 0.05 ） in 21 th day after weaned; M OS increased the concentration of IgM profoundly（ P 〈 0.05 ） on the 21 st day after weaning. （3）In 7th day after weaning, the percentages of CD4^＋ of thymus, spleen, lymph node in group FOS （37.33 ± 4.04, 30.33± 8.73, 37.67 ±2.52） were higher than that in group ABT（21.33 ± 6.03, 19.67 ± 2.08, 24.67 ±4.16 accordingly） （ P 〈 0.05 ） , the percentages of CD8^＋ of spleen in group F ＋ M （22.66± 3.05 ） were higher than in group ABT（ 17.00±1.00） （ P 〈 0.05 ）, the percentages of CD8^＋ of thymus in group MOS（29.67± 5.51 ）were higher than that in group ABT（20.67 ± 3.51 ） , the percentages of CD8^＋ of lymph node in group F ＋ M （20.66 ± 6.42） were lower than that in group ABT（ 34.33 ±5.03） （ P 〈 0.05 ） ; On the 28th day after weaning, the percentages of CD4^＋ of thymus in group FOS（ 32.00 ± 5.29 ） , MOS（ 37.33 ± 3.21 ）, F ＋ M （ 32.00 ± 3.60 ） were higher than in group ABT（21.33 ± 4.04） （ P 〈 0.05 ） , there were no significant differences among group FOS, MOS, F ＋ M and antibiotics（ P 〉 0.05） in CD8^＋ of thymus, spleen and lymph node（ P 〉 0.05） ; Though there were no significant differences among group FOS, MOS, F ＋ M and ABT, CD4^＋/CD8^＋ ratio in group FOS, MOS, F ＋ M was larger than that in group ABT, except that lympho node CD4^＋/CD8^＋ ratio in group FOS （ 1.42 ± 0.32） , MOS （ 1.52 ± 0.46）, F ＋ M （ 1.51 ±0.30） was larger （ P 〈 0.05 ） than that in group ABT（0.71 ± 0.03 ） on the 7th day after weaning and spleen CD4^＋/CD8^＋ ratio in group FOS（ 1.36 ± 0.21 ）, MOS（ 1.34 ±0.16） was larger（ P = 0.01 ） than that in group ABT（0.94±0.29）.