摘要
目的 K-ras基因的突变激活,最常见于胰腺癌、结肠癌和肺癌等肿瘤。国外有人报道人喉鳞状细胞癌的发生与K-ras基因的突变激活没有相关性。本文旨在探讨K-ras基因在喉鳞状癌细胞株Hep-2中的表达及其意义,为喉癌发生与K-ras点突变的相关性研究奠定坚实的基础。方法采用RT-PCR技术检测K-ras基因mRNA在喉癌细胞株Hep-2和胰腺癌细胞株MIAPaCa-2中的表达。结果人胰腺癌细胞株MIAPaCa-2和人喉鳞状癌细胞株Hep-2中K-ras mRNA表达均为强阳性。结论人喉鳞状细胞癌细胞株Hep-2中K-ras基因表达阳性,喉癌的发生可能与其点突变引起的激活有关。
Objective： To investigate the expression of K-ras in human laryngeal squamous cell carcinoma cell lines （Hep-2） and its significance for establishing a solid foundation for further study of the relationship between human laryngeal squamous cell carcinoma and K-ras gene point mutations. Methods： The expression of K-ras in human laryngeal squamous cell carcinoma cell lines （Hep-2） and human pancreatic carcinoma cell lines （MIAPaCa-2） was detected by using RT-PCR. Results： The expression of K-ras mRNA in Hep-2 and MIAPaCa-2 was strong and positive. Conclusion： The expression of K-ras mRNA in human laryngeal squamous cell carcinoma cell lines （Hep-2） is positive. Development of laryngeal carcinoma might be related to the activation of K-ras gene point mutation.
基金
This work was supported by a grant from the National Natural Science Foundation of China(No. 30070809).
