Detection of K-ras Gene Point Mutation＇s Style in Human Pancreatic Cancer Cell Line PANC-1 by PCR-SSP

客观：在人的胰腺的癌症房间线 PANC-1 检测 K 地岬基因点变化的风格；确定地岬目标位置的 bp 顺序由 RNA 介入了。方法：为关于变化式样的聚合酶链反应(PCR ) 的三种特殊顺序教材(SSP )(GAT，法国总工会；GGT ) 在 12 K 地岬上的鳕鱼被用来学习人的胰腺的癌症房间线 PANC-1。扩大产品与 polyacrylamine 胶化电气泳动被学习检测点变化的风格。结果：在 12 上的鳕鱼的 K 地岬基因点变化的风格是在人的胰腺的癌症房间线的 GAT。结论：PCR-SSP 是快速的，方便；高特定。结果为胰腺的癌症由 RNA 干扰为进一步的基因治疗提供一个基础。

Objective： To detect the style of K-ras gene point mutation in human pancreatic cancer cell line PANC-1 and decide the bp sequence of Ras target position interfered by RNA. Methods： Three kinds of special sequence primers （SSP） for polymerase chain reaction （PCR） with regard to the mutation styles （OAT, COT and GOT） at codon 12 of K-ras were used to study the human pancreatic cancer cell line PANC-1. The amplification products were studied with polyacrylamine gel electrophoresis to detect the style of point mutation. Results： The style of K-ras gene point mutation at codon 12 was OAT in human pancreatic cancer cell line. Conclusion： PCR-SSP is rapid, convenient and high specific. The results provide a basis for further gene therapy by RNA interference for pancreatic cancer.