黄淮麦区小麦品种(系)的ISSR位点遗传多样性分析

Genetic Diversity of ISSR Loci for Wheat Cultivars of Huang-Huai Winter Wheat Region

选用11个ISSR引物，对黄淮麦区96个小麦推广品种（系）进行遗传多样性分析。共检测到96个多态性位点，每个引物多态性位点数平均为8．7个，变幅为3～23个；ISSR引物的多态性信息含量PIC变幅为0．601～0．941，平均0．791，表明ISSR具有较强的品种间区分能力，是研究小麦种质资源遗传多样性的有效分子标记技术之一。96个品种（系）间，Nei’s遗传相似系数变化范围为0．53～0．91，平均为0．60，品种间遗传相似性变幅较大，表明黄淮麦区不同小麦品种（系）间存在着不同程度的遗传多样性差异。根据品种间遗传相似系数聚类，96份材料被聚成8大类群，共14个亚类，类群与系谱和原产地无关。

ISSR （inter simple sequence repeat） markers were used to detected genetic diversity among wheat cultivars （or breeding lines） of Huang-Huai Winter Wheat Region of China. Totally 96 alleles of 11 ISSR markers were detected among 96 cultivars with the average of 8.7 alleles per ISSR marker, and the number of alleles per primer ranged from 3 to 23. The value of allelic polymorphism information content （PIC） ranged from 0.601 to 0.941, on the average of 0.791 per primer. These results showed that ISSR is a suitable molecular technique for genetic diversity analysis of wheat germplasm resources. The value of Nei＇ s genetic similarity （GS） indexes of 96 cultivars based on the ISSR data varied from 0.35 to 0.91, with an average of 0.60, indicating that the genetic diversity among cultivars of Huang-Huai Wheat Region is abundant. Cluster analysis showed that the cultivars tested in this study could be clustered into eight groups and fourteen subgroups, which generally do not agree with pedigree relations and regions of origin.