ELISA检测抗精子抗体并与间接血凝法比较

DETECTION OF SPERM ANTIBODI ES BY ELISA AND COMPARISON WITH THE INDIRECT HEMAGGLUTINATION TEST

本文结果表明,188份结扎兔血清中,ELISA抗精子抗体阳性者为85.1％,间接血凝(IHT)为62.8％,差异显著(P<0.005),以118份IHT阳性血清的抗体滴度对数值与相对应的ELISA光密度值相关分析结果表明,二者有非带显著的正相关(P<0.001)。我们认为,ELISA检测抗精子抗体是一种灵敏度高、方便、迅速且易于操作的免疫学方法。

The results in this paper showed that the positive rate for antisperm antibodies in 188 sera of vasectomized rabbits was 85.1% by ELISA and 62.8% by the indirect hemagglutination test (IHT) (P<0.005) .Positive correlation was found between ELISA O.D.and IHT titres.The results indicate that ELISA is a sensitive, rapid and convenient method for detection of sperm antibodies.