摘要
目的:比较三种荧光定量PCR检测仪的偏倚。方法:采用实时荧光定量PCR,随机选择我院门诊和住院病人标本42例,用同一种试剂抽提DNA摸板后,分别在PE7000、伯乐及罗氏定量PCR扩增仪平行测定。结果42份样品在三种PCR检测仪上所测定HBVDNA拷贝/ml对数均值分别为5.625±1.449、5.420±1.960、5.803±1.536,三组之间比较无差异(P>0.1)。以测定拷贝数值≤或≥5.0×105/ml将其分为二组,在标本HBVDNA含量≤5.0×105拷贝/ml,伯乐测定值偏低,与罗氏比较差异有显著性意义((P<0.05))。结论伯乐仪器对HBVDNA低拷贝含量标本检测值偏低,PE7000型仪器有操作简单、耗材低廉、检测数据稳定及价格适中等优点,更适合于临床。
Objective： To evaluate PE700/ABI and icycler/BioRad and Lightcycler/Roche. three fluorescent quantitative machines. Methods： Specimens were analyzers with one reaget by the three fluorescent quantitative machiines. Results： all the results obtained from the three machines were not significant. But 23 patients with the HBV-DNA value less than 5^＊ 10^5 copies/ml were significant difference between icycler/BioRad and Lightcycler/Roche .conclusion among of them PE7000 is much better.
出处
《中国医疗器械信息》
2006年第2期40-40,49,共2页
China Medical Device Information
关键词
荧兆定量PCR仪
应用评价
Fluorescent Quantitative Machine, Evaluation Studies
