甲醛吸入染毒对小鼠DNA损伤的影响

Effects of formaldehyde inhalation on DNA damage in mice somatic cells

目的探讨甲醛吸入染毒对小鼠脾、肝、肺和肾组织细胞DNA的损伤作用。方法将40只健康昆明种小鼠随机分为5组,混合物静式吸入染毒,用不同剂量(0.0、0.75、1.5、3.0、6.0mg/m3)的甲醛染毒小鼠2周,第15天处理动物后,取脾、肝、肺、肾组织制成细胞悬液,用单细胞凝胶电泳实验观察其细胞DNA损伤水平。结果染毒后小鼠脾、肝、肺、肾组织细胞DNA出现损伤,且损伤程度与染毒剂量具有一定相关性。与空白对照组比较,3种组织细胞各剂量组细胞DNA拖尾率和彗星尾长均显著增加;随着甲醛染毒浓度的升高,脾、肝、肺和肾细胞拖尾率和彗星尾长增加。结论甲醛可引起小鼠的脾、肝、肺、肾组织细胞DNA损伤。

Objective To study the effect of formaldehyde inhalation on nuclear DNA damage in mouse somatic cells using single cell gel eletrophoresis（SCGE） comet assay. Methods 40 mice were randomly divided into 5 groups consisting of 8 mice per each group. Static inhalation was applied to mice exposed to the formaldehyde at different concentrations（0.0, 0.75, 1.5,3.0, 6.0mg/m3） .Mice were sacrificed by cervical dislocation 2 weeks after treatment and their spleen, liver, lung and kidney were rapidly excised and homogenized , and DNA damage in their somatic cells was tested by SCGE comet assay. Results DNA of spleen, liver, lung and kidney cells was significantly damaged after exposure, and there were some correlation between exposure concentration and damage degree. Comparing to control group, significant increasing was observed in the percentage and with mean tail length of cells with tails in each groups treated with various doses of formaldehyde; The percentage and with mean tail length in above-mentioned cells also increased as. formaldehyde dose increased. Conclusion Inhalation of formaldehyde could lead to DNA damage in mice spleen ,liver,lung and kidney cells.