人参皂甙对β淀粉样蛋白诱导THP-1单核细胞一氧化氮合酶和一氧化氮的影响

Effects of Ginsenoside on Expression of Nitric Oxide Synthase and Concentration of Nitric Oxide in THP-1 Cells Induced by β-amyloid

目的研究人参皂甙对β淀粉样蛋白(βamyloid,Aβ)1-40诱导THP-1细胞表达诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)和一氧化氮(nitric oxide,NO)的影响。方法用Western blotting测定THP-1细胞iNOS表达,以Griess法测定细胞上清中NO的浓度。结果Aβ模型组iNOS的表达明显高于对照组(P<0.01)。与模型组比较,人参皂甙可明显减少THP-1细胞iNOS的表达和NO的产生(P<0.05)。随着人参皂甙剂量的增大,人参皂甙可引致THP-1细胞iNOS的表达和NO的产生明显减少(P<0.05),且有剂量依赖性。结论人参皂甙可抑制THP-1细胞iNOS的表达,进一步影响NO的产生。

Objective To investigate the effects of ginsenoside （GS） on expression of inducible nitric oxide synthase （iNOS） and concentration of nitric oxide （NO） in THP-1 cell line induced by β-amyloid （Aβ）1-40. Methods The concentration of nitric oxide was determined by Griess reagent in supernatant of THP-1 cells induced by Aβ. The content of iNOS was measured by Western blotting. Results expression of iNOS and concentration of NO in Aβ model group were significantly higher than those in control group, while the GS treatment reduced expression of iN（）S and concentration of NO obviously in THP-1 cells induced by Aβ. Compared with model group, expression of iNOS and concentration of NO were remarkably lower. Ginsenoside causes dose-dependent decrease in expression of iNOS and concentration of NO. Conclusions GS significantly inhibited expression of iNOS and reduced secretion of NO in THP-1 monoeytes induced by Aβ. GS might provide a novel therapy for AD.