大鼠脑出血灶周围区内和血浆中干扰素-γ基因及蛋白的表达

Expression of Iterferon-gamma in Perihematomal Brain Region and Plasma after Experimental Intracerebral Hemorrhage in Rats

目的观察大鼠脑出血血肿周围组织和血浆中干扰素-γ(IFN-γ)基因及蛋白的变化。方法采用Ⅶ型胶原酶立体定位法制备大鼠尾状核出血动物模型,以逆转录多聚酶链反应(RT-PCR)检测血肿周围组织IFN-γmRNA的表达,采用免疫组化法和酶联免疫吸附法(ELISA)观察脑出血灶周围区和血浆中IFN-γ基因及蛋白在出血后不同时间点的表达。结果脑出血后血肿周围IFN-γ反应阳性细胞和IFN-γ浓度于发病早期(4 h)即明显升高,3 d达峰值并持续至7 d。结论IFN-γ可能参与脑出血后的继发性损伤以及脑出血后迟发性细胞免疫损伤。

Objective To observe the expression of interferon gamma（IFN -γ）in rat perihematomal regions and blood after experimental haemorrhagic stroke. Methods Heamorrhagic stroke rat model made by injecting collagenase Ⅶ stereotaxically. Reverse transcription-polymerase chain reaction were used to measure expression of IFN--γmRNA in perihematomal tissures; the level of IFN-γprotion were measured dynamically by immunohistochemically （SP meathods） and enzyme-linked immunosorbent assay in perihematomal fissures and blood. Results IFN--γmRNA and IFN--γprotion had significant increase in early phase （4 hour） and reached to top level in third day and sustained for 7 days in pcrihematomal regions and plasma after experimental heamorrhagic＇ stroke. Conclusions These results indicate that IFN--γmay take part in the secondary damage and is possible to play an important role in the delayed cell immunity damage after experimental heamorrhagie stroke.