一种新的蝮蛇蛇毒磷脂酶A2同源物的分离纯化及其氨基酸序列分析

Purification of A New Phospholipase A2 Homologue from Agkistrodon blomhoffii Siniticus and Its Effects on Gene Expression of Hep3B Cells

采用C18反相高效液相色谱法从蛇毒粗毒中提取出一种新的PLA2同源物,其分子量为13900Da,N末端氨基酸序列分析结果为:HLLQFRKM IKKMTKK。通过GenBank的prote in-prote in BLAST软件进行同源性分析,结果表明,N端的15个氨基酸与已知其他蛇毒PLA2具有较高的同源性。

A new PLA2 homulogue was purified ti＇om Agkistrodon blomhoJyd Sinlticus by applying reverse phase （HPLC） C18 column. The molecular weight of PLA2 hnmologue is 13900Da and its purity is 97.2%. The results of N-terminal sequence analysis were HLLQFRKMIKKMTKK. All the fragments were determined with the protein-protein BLAST software of GenBank. BLAST analysis showed that the N-terminal sequences of 15 amino acids were highly homologous with the sequences of other PLA2.