猪创伤皮肤缺损修复实验中的麻醉研究

Anaesthesia Research in Process of Experiment of Porcine Traumatic Skin Defect and Repair

目的探索一种适合猪创伤皮肤缺损修复实验的麻醉方法。方法实验分两部分,一是创伤皮肤缺损和修复动物模型制作手术中的麻醉,将14只仔猪随机分为3组,分别采用气管插管呼吸机辅助呼吸氯胺酮、安定、芬太尼复合麻醉、氯胺酮肌注加水合氯醛静滴麻醉、氯胺酮加戊巴比妥钠腹腔注射麻醉。二是换药中的麻醉,将64次麻醉分为3组,分别采用单纯氯胺酮麻醉、单纯戊巴比妥钠腹腔、氯胺酮加戊巴比妥组肌注。观察显效时间、维持时间、呼吸频率、心跳频率、麻醉效果。结果皮肤创伤缺损和修复动物模型制作手术中,3组麻醉比较结果显示气管插管呼吸机辅助呼吸氯胺酮、安定、芬太尼复合麻醉显效时间快,维持时间可以控制,麻醉效果最好,安全可靠。在换药的麻醉中,氯胺酮加戊巴比妥组肌注显效时间比较快,麻醉时间较短,效果好,动物容易苏醒。结论创伤皮肤缺损和修复动物模型制作手术中,采用气管插管呼吸机辅助呼吸氯胺酮、安定、芬太尼复合麻醉是有效安全的麻醉方法;在换药的麻醉中,氯胺酮加戊巴比妥组肌注是较好的麻醉方法。

Objective To explore a kind of method which is suitable for the experiment of making the model of porcine traumatic skin defect and repair. Methods The experiment divided into two parts. The first part was the anaesthesia in the operation of making the model of porcine traumatic skin defect and repair. 14 piglets were divided into three groups at random. Group 1 ： Adopling the trachea intubatc assist breath with breathing machine, and compound anaesthesia with katamine hydrochloride, valium, fentanyl citrate; Group 2： Injection of katamine hydrochloride into muscle and chloral hydrate drip by vein; Group 3： Injection of katamine hydrochloride into muscle and pentobarbitital sodium into abdominal cavity. The second part was the anaesthesia in the process of change dressings. 64 times change dressings were divided into three groups： Group 1： Injection of katamine hydrochloride simply; Group 2： Injection of pentobarbitital sodium into abdominal cavity simply; Group 3： Injection of katamine hydrochloride and pentobarbitital sodium into muscle. The time showing effect, nlaintain time, frequence of respiration and heartbeat and anaesthetic effect were observed and analysed among the groups. Results In part 1, The resuh showed that the anaesthetic effect of Group 1 was the best, the time showing effect was the shortest, the maintain time control easily and also it was the most safety one in the Group 1. In part 2, the time showing effect and the maintain time were shorter and the anaesthetic effect was the best in the Group 3. In addition, the animal was apt to revive in tbc Group 3. Conclusion Compound anaesthesia with katamine hydrochloride, valium, fentanyl citrate and adopting the trachea intubate assist breath with breathing machine is an effective and safe method in the operation of making the model of porcine skin traumatic defect and repair. The anaesthesia which injection of katamine hydrochloride and pentobarbitial sodium into muscle is a better methot in the process of change dressings.