大丽轮枝菌粗毒素在棉花根冠细胞生物学测定方法上的研究

Several Improvements for Bioassay of Verticillium dahliae Kleb Coarse Toxin in Cotton Root Cap Cell

在棉花根冠细胞生物测定过程中，棉花黄萎病病菌通过提纯复壮而达到较强的侵染能力，并制备出较多的黄萎病菌粗毒素。介绍了粗毒素的简易制备、根冠细胞培养与制备过程以及染色过程中染色剂的选择、染色时间的长短。实验与传统方法相比有几点改进：总结出细胞振荡时间在1～2min之内；之后黑暗条件下静置培养2～6h；选用pH为6．5的0．01％中性红染色3～5min，之后加入一滴0．2％伊文思兰染色，伊文思兰的染色时问应严格掌握在3min以内等，为根冠细胞测定方法的推广提供了一定的理论支持。

Several problems for attention to the bioassay of cotton root cap cell with Verticillium dahliae Kleb coarse toxin were studied. The text compared with the old way： The shaking time for collecting the root cap cell should be controlled in 1 - 2 min; Root cap cell in the culture chamber with darkness was taken 2 - 6 h; The natural red （pH = 6.5, 0.01% for 3～5 min）and Evans blue（0.2 % for 3min）as dye stuff were used. The results indicated that with analyzing living cell of root cap treated by Verticillium dahliae Kleb toxin was a quick way in the room to identify the disease resistance of different cotton varieties.