蛇床子和白芷中香豆素类化合物抑制磷酸二酯酶5活性的研究

Inhibitation of Coumarins in Cnidium monnieri Cusson and Angelian dahuricn on PDE5

目的研究香豆素类化合物的PDE5抑制活性。方法用体积分数为95％乙醇和氯仿分别提取蛇床子及白芷中总香豆素类化合物,并通过柱色谱和HPLC进行分离,结构鉴定后进行PDE5抑制活性研究。结果分离得到8个化合物,结构修饰4 个化合物,并测得其PDE5抑制活性数据。结论蛇床子素具有较强的PDE5抑制剂。

OBJECTIVE To study the activity of coumarins as PDF25 inhibitor.METHODS The coumarins were extracted with 95% alcohol from Cnidium monnieri （L.） Cusson and with CHCl3 from Angelica dahurica Benth. et Hook., and then isolated via column chromatography and HPLC. After structure identification, the inhibition of compotmds on PDE5 was investigated according to the existing methods. RESULTS 8 compotmds were isolated and identified, and 4 compounds were obtained by structure, modification. The activities data as PDE5 inhibitors were tested. CONCLUSION Osthol showes the potent inhibitation on PDE5.