卡巴胆碱对肠缺血-再灌流动物脏器功能的保护作用

Protective effects of carbachol on organ dysfunction induced by gut ischemia-reperfusion in animals

目的研究卡巴胆碱对肠缺血再灌流动物脏器功能的保护作用及对肠组织和白细胞炎症介质表达的影响。方法检测肠缺血再灌流大鼠和肠部分缺血再灌流兔伤后脏器功能指标、白细胞中细胞因子表达和血浆、肠组织TNFα含量。结果卡巴胆碱治疗组大鼠再灌流2h后血浆ALT、BUN和CK低于对照组,白细胞中TNFα表达下调,IFN、IL4和IL10表达上调;治疗组兔血浆ALT、Cr和CKMB在缺血和再灌流早期增加幅度低于实验组,再灌流后趋于恢复,实验组渐升高;肠组织TNFα含量低于实验组。结论卡巴胆碱改善肠缺血再灌流动物脏器功能,抑制肠组织TNFα的合成,调节白细胞致炎和抗炎因子的表达比例。

Objective To investigate the protective effects of carbachol on organ dysfunction induced by gut ischemia-reperfusion and on the synthesis and expression of inflammatory cytokines in the intestinal tissue and the leukocytes. Methods Models of superior mesenteric artery obstruction （SMAO） in rats and superior mesenteric artery partial obstruction （SMAPO） in rabbits were adopted. The indicators of organs function, mRNA expression of cytokines in leukoeytes and TNFα contents in the intestinal tissue and the blood were detected at different time points. Results in SMAO group, the plasma ALT, BUN and CK of rats treated with carbachol were lower than those in rats without earbchol at 2 hours after repeffusion; the mRNA expression of TNFα in leukocytes was down-regulated and those of IL-4, IL-10 and IFN were up-regulated in rats treated with carbachol. In SMAPO group, ALT, Cr and CK-MB in the plasma increased during isehemia and at early reperfusion stages in rabbits treated with earbachol, but the enhanced extent was less than those without carbachol, and returned to the normal during 1 to 3 days after reperfusion; the content of TNFα in the intestinal tissues of rats with carbaehol was less than that without carbachol. Conclusion Carbachol could improve liver, kidney and heart dysfunction, inhibit the synthesis of TNFα in the intestinal tissues, and regulate the expressive proportion of pro- and anti- inflammatory cytokines.