摘要
目的观察转化生长因子-β1(transforming growth factor-β1,TGF-β1)诱导人视网膜色素上皮细胞(retinal pigment epithelium,RPE)凋亡的作用机制。方法使用逆转录多聚酶链反应检测不同浓度TGF-β1作用下的人RPE细胞中凋亡相关基因bcl-2和FasmRNA的表达,使用Western-Blot蛋白印迹法检测不同浓度TGF-β1处理过的PRE细胞中Caspase-3蛋白的表达。结果逆转录多聚酶链反应显示,随着TGF-β1浓度的增高,人RPE细胞中bcl-2mRNA表达降低,Fas mRNA的表达逐渐增高;Western-Blot结果显示,随着TGF-β1浓度的增高,Caspase-3蛋白表达逐渐增高。结论TGF-β可上调RPE细胞表面Fas基因的表达,从而激活Caspase-3的活性而诱导RPE细胞凋亡,Caspase-3在RPE细胞凋亡过程中起着非常重要的作用。
Objective To investigate the mechanism of transforming growth factor-β1 （TGF-β1） on inducing apoptosis in cultured human retinal pigment epithelium （hRPE） cell.Methocls Reverse transcription polymemerase reaction（RT-PCR） was used to detect the mRNA expression of bcl-2 and Fas by different concentration TGF-β1 In hPRE cell. Western-Blot was used to detect the protein expression by different concentration TGF-β1 in hPRE cell.Results RT-PCR showed that the TGF-β1 concentration was directly proportional to the Fas mRNA expression. The mRNA expression of bcl-2 was gradually reduced in hPRE cell when the TGF-β1 concentration was gradually increased. Western-Blot showed that TGF-β1 concentration was directly proportional to the Caspase-3 peotein expression,Conclueion TGF-β1 may up-regulate the expression of Fas mRNA and activate the activity of Caspase-3 protein. Caspase-3 plays an importance role in the process of RPE cells apoptosis. [Rec Adv Ophthalmol 2006 ;26（3） ： 194-197]
出处
《眼科新进展》
CAS
2006年第3期194-197,共4页
Recent Advances in Ophthalmology
