亚甲基四氢叶酸还原酶基因C677T多态与肝细胞癌遗传易感性的相关性研究

A study on the association of MTHFR C677T polymorphism with genetic susceptilbility to hepatocellular carcinoma

目的探讨亚甲基四氢叶酸还原酶(MTHFR)基因C677T多态与中国人肝细胞癌(HCC) 遗传易感性的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR—RFLP)方法,检测508例 HCC与543例对照的MTHFR C677T基因型分布及其差异。结果 HCC和对照两组人群的MTHFR C677T 基因型分布差异无统计学意义。但与C等位基因携带者(C／C和C／T基因型)相比,T／T基因型携带者患 HCC的风险增加0．66倍(95％可信区间为1．08～2．54,P＜0．05)。性别因素分层分析结果显示：T／T基因型女性携带者其HCC的风险是C等位基因女性携带者的2．64倍(95％可信区间为1．19～5．88,P＜0．05)；而男性T／T基因型与C等位基因携带者其HCC的风险差异无统计学意义。结论 MTHFR基因C677T多态可能是中国女性患HCC的一个遗传易感因素,而男性HCC发病风险与该多态无明显关系。

Objective To evaluate the possible association of the MTHFR C677T polymorphism with genetic susceptibility to hepatocellular carcinoma （HCC） in a Chinese population. Methods Five hundred and eight HCC cases and 543 controls were studied. The MTHFR genotypes were determined using a PCR-based restriction fragment length polymorphism （RFLP） method. Odds ratios （ORs） for HCC and 95% confidence intervals （CIs） from unconditional logistic regression models were used to evaluate relative risks. Potential HCC risk factors were included in the logistic regression models as covariates in the multivariate analyses on genotypes and HCC risks. Results No overall significant difference in genotype distribution was found when comparing all HCC cases to controls （P = 0.258）. However, a significantly increased risk of HCC was observed among T/T homozygotes （adjusted OR = 1.66, 95% CI = 1.08-2.54, P 〈 0.05） compared to C-allele carriers （CC or CT）. When stratified with sex, this trend was more prominent in females, but not in males. Females who were homozygous （T/T） for the C677T polymorphism were at a 2.64-fold （95% CI = 1.19-5.88, P 〈 0.05） increased risk of developing HCC when compared to C-allele carriers. However in males, T/T homozygotes had a similar risk with C-allele carriers. Conclusion The MTHFR C677T polymorphism may be associated with a higher HCC risk in females, but not in males in this population.