摘要
目的研究胃癌患者癌组织中人疱疹病毒4型(EB病毒,epstein-Barr virus,EBV)感染与B细胞淋巴瘤或血血病-2基因(B cell lymphoma/leukemia-2,bcl-2)表达的关系。方法原位杂交法(In Situ Hybridization,ISH)检测胃癌患者石蜡标本中EBV编码的小RNA(EBER1);免疫组化法(immunohistochemistry,IHC)检测EBV阳性胃癌及EBV阴性胃癌标本癌基因bcl-2的表达状况。结果217例胃癌患者癌组织标本中检测到23例呈EBER1阳性;EBV阳性胃癌组Bcl-2蛋白表达显著高于EBV阴性胃癌组,平均面积(avevage area,AA)、平均光密度(mean opticaldensity,MOD)和积分光密度(integral optical density,IOD)分别为(67.31±5.95)μm2、17.60±2.80和1.75±0.13 vs(38.1±4.14)μm2、5.000.0和1.35±0.07(P<0.01)。结论EBV感染可能上调了癌基因bcl-2表达。
Objective To investigate the influence of EB virus（EBV） infection on the expression of oncogene bcl-2. Methods EBER1 in 217 cases of gastric carcinoma were detected with in situ hybridization. Then, EBV positive and negative cases were selected for analysis of the expression of bcl-2 by immunohistochemistry. Results In 217 eases of gastric carcinoma, there were 23 cases with EBER1 positive. The expression of bcl-2 was higher in EBV positive gastric carcinoma than that in EBV negative, respectively AA, MOD and IOD（67.31 ± 5.95）μm^2 , 17.60 ± 2.80 and 1.75±0.13 vs （38.1±4.14）μm^2,5.000.0 and 1.35±0.07（P 〈0.01）. Conclusion EBV infection in gastric carcinoma increased the expression of bcl-2.
出处
《临床荟萃》
CAS
北大核心
2006年第5期319-321,F0002,共4页
Clinical Focus
基金
教育部留学回国人员科研启动基金(编号教外司留[2000]479)
