未获培养的微生物与牙周病关系初探

Detection the Association between Uncultivated Phylotypes and Periodontitis

目的:研判未获培养微生物与牙周病的相关性。方法:针对16S rRNA基因设计未获培养微生物D084、I025特异探针,通过检测临床菌斑判断其与牙周病的关系。结果:引物特异性通过PCR产物测序获得验证,在慢性牙周炎组和单纯性龈炎组的D084检出率分别为62.9%和84.6%,I025为71.4%和42.3%,与正常组均有显著性差异(P<0.01)。I025在慢性牙周炎组和单纯性龈炎组间的检出率也存在显著性差异(P<0.05)。结论:未获培养微生物D084、I025与单纯性龈炎、慢性牙周炎患病相关,I025还与牙周炎进展相关。

Objective： To discuss the relationship between uncultivated pathogenic bacteria and periodontitis. Methods： Specific PCR primers were designed for phylotypes D084 ＆ I025. Polymerase chain reactions were applied to determine the prevalence of these phylotypes in chronic periodontitis, plaque- induced gingivitis and healthy control subjects. Results： The specificity of each primer was validated on the basis of sequence analysis of PCR products. D084 ＆ 1025 were detected in subgingival plaque samples in all three groups. The prevalence of D084 in subgingival plaque in chronic periodontitis （62.9%） and plaqueinduced gingivitis （84.6%） was relatively higher than that in healthy ones, the difference was found statistically significant （P 〈0.01 ）. The prevalence of 1025 in subgingival plaque in periodontitis patients （71.4%） and plaque - induced gingivitis patients （42.3%） were higher than that in healthy ones, the difference was statistically significant （P 〈 0.01 ）. The difference between chronic periodontitis group and plaque - induced gingivitis groups was statistically significant （P 〈0.05）. Conclusion： The novel uncultivated D084 and I025 phylotypes could be related to chronic periodontitis and plaque - induced gingivitis. I025 might play a role in the progress of lesion from gingivitis to periodontitis.