摘要
目的探讨β-榄香烯(-βelemene)对多药耐药细胞株K562/阿霉素(ADM)的耐药逆转及其对该细胞P-糖蛋白(P-gp)表达的影响。方法采用MTT法检测细胞的药敏性及耐药逆转,免疫组织化学及免疫电镜观察P-gp的表达,流式细胞术进行定量分析,应用SPSS(10.0 production pacility)软件包对实验结果进行统计学处理。结果1.非细胞毒性浓度的-βelemene(4.0 mg/L)可显著降低ADM对K562/ADM细胞的IC50(P<0.01),逆转倍数为2.18倍;2.P-gp在耐药细胞株K562/ADM中呈高表达,而在敏感细胞株K562中表达很低,进一步定位研究结果表明,P-gp主要分布于细胞膜上;3.非细胞毒性浓度的-βelemene(4.0 mg/L)能够显著降低耐药细胞P-gp的表达。结论-βelemene能够明显逆转K562/ADM细胞对ADM的耐药性,降低该细胞P-gp的表达是其主要逆转机制之一。
Objective To explore the effect of β-elemece on the reverse of drug resistance and the expression of P- glycoprotein （P-gp） in MDR K562/ADM cells. Methods MTT method was used to detect the sensitivity of cell and its reverse of drug resistance. Immunohistochemistry and immunoelectron microscopy were for observing the expression of P-gp. It＇s quantitative analysis was determined by flow cytometry. All experimental data were dealt with by SPSS （10.0 production pacility） soft ware. Results 1.Non-cytotoxic dose of β-elemece （4.0 mg/L） could obviously decrease the IC50 value of K562/ADM cells to ADM. The reversing fold was 2.18 ;2. The level of P-gp expression was higher in K562/ADM cells than that in its parent K562 cells. Our studies also showed that the distribution of P-gp was on membrane of K562/ADM cells;3.Non-cytotoxic dose of β-elemece （4.0 mg/L） could remarkably decrease the P-gp expression in MDR K562/ADM cells. Conclusion β-elemece could evidently reverse drug resistance of K562/ADM to ADM. Decreasing the P-gp expression in drug resistance cell is one of the main mechanism of
出处
《解剖学报》
CAS
CSCD
北大核心
2006年第1期48-51,共4页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(39170853)
