树突状细胞标志分子在人乳腺癌中的表达及与T细胞的关系

EXPRESSION OF DENDRITIC CELL MOLECULE MARKER AND ITS RELATION WITH T CELL IN HUMAN BREAST CANCER

目的通过观察人乳腺癌组织中CDlc、S-100、HLA-DR阳性细胞数和表达强度的变化及其与T细胞的关系,了解乳腺癌局部微环境的免疫状态,探讨树突状细胞与乳腺癌发生、发展的关系,为乳腺癌的生物治疗提供实验依据。方法收集手术切除的人乳腺癌组织和癌旁相对正常乳腺组织,用免疫组织化学方法和图像分析技术对20例乳腺癌标本进行检测。结果乳腺癌组织中CDlc、S-100和HLA-DR的表达强度均较相对正常乳腺组织明显减弱(P<0.05);CD1c+DC、S-100+DC、HLA-DR+DC、CD4+T细胞和CD8+T细胞的数量均较相对正常乳腺组织明显减少(P<0.05),且乳腺癌组织中CDlc+DC细胞的数量比S-100+DC细胞的数量减少更明显,HLA-DR+DC与CD4+T细胞的数量呈明显相关关系(r=0.998);结论树突状细胞表面标志分子在人乳腺癌组织中的表达总量下降,树突状细胞在抗乳腺癌的免疫反应中可能起重要作用;这为临床开展乳腺癌的生物治疗提供了实验依据。

Objective To investigate the numbers and distribution of dendritic ceils and the expressive intensity of CDlc, S-100 ,HLA-DR and its relation with T cell in human breast cancer tissues, and to discuss the relationship between dendritic cell （DCs） and developing of breast cancer. The study can provide the experimental data in the further biological therapy of breast cancer. Methods The human breast cancer tissues and relatively normal breast tissues were collected from surgical operation and the samples of 20 patients with breast cancer were analyzed by immunocytochemistry and computer imaging techniques. Re.suits The result showed that the expressive intensity of CDlc ,S-100, HLA-DR was dramatically weaker in breast cancer tissues than in those of relatively normal breast tissues（ P 〈 0.05）. The number of CDlc^＋ DCs, S-100^＋ DCs, HLA-DR^＋ DCs,CD4^＋ T ceils and CD8^＋ T cells reduced greatly with significance（ P 〈 0.05） .The number of CDlc^＋ DCs reduced more than that of S-100^＋ DCs in breast cancer tissues. There was a linear correlation between the number of HLA-DR^＋ DCs and CD4^＋ T cells（ r = 0.99g）. Conclusion The total expression of dendritic cell molecule marker was reduced in breast cancer, which may play an important role in initiating immunity against breast cancer. The result might be very useful for the further clinical biological therapy of breast cancer.