摘要
mRNA差异显示逆转录PCR技术是一种在转录水平上研究基因差异表达的方法,该方法已成为医学真菌分子生物学研究的良好工具。运用该技术已经寻找到白念珠菌、曲霉等真菌的一些致病相关基因。并有助于了解系统性真菌病的致病机制、开发新的抗真菌药物、真菌疫苗的研制、真菌病的早期诊断和早期治疗。
mRNA differential display reverse transcription-PCR （DDRT-PCR） is a technique to study the difference between gene expression at the level of transcription. It has become an efficient tool for molecular biology research of medically important fungi. Some genes related to the pathogenicity of Candida albicans, Aspergillus and other fungi have been found by this technique. It will help to understand the pathogenesis of systemic mycoses, to develop new antifungal agents, to prepare fungal vaccines and to make early diagnosis and treatment of mycoses.
出处
《国际皮肤性病学杂志》
2006年第2期87-89,共3页
International Journal of Dermatology and Venereology
