雌激素对血清饥饿诱导成骨细胞凋亡的影响

Effect of Estrogen on Osteoblast Apoptosis Induced by Serum Hungry

目的探讨雌激素对血清饥饿诱导体外培养大鼠成骨细胞凋亡的影响。方法新生SD大鼠颅骨第2、3代成骨细胞随机分为对照组、血清饥饿组和血清饥饿+雌激素组,每组细胞分别培养24h、48h、72h、5d、7d、14d后行TUNEL染色观察凋亡细胞的形态特征;流式细胞仪检测各组细胞的凋亡率。结果对照组细胞核未见明显异常;血清饥饿组可见细胞核内有紫兰色颗粒的凋亡细胞;血清饥饿+雌激素组凋亡细胞明显减少;与对照组比较,血清饥饿组细胞凋亡率升高,血清饥饿+雌激素组细胞凋亡率降低(P<0.05)。结论雌激素能抑制血清饥饿诱导的大鼠成骨细胞凋亡。

Objective To explore the effect of estrogen on osteoblast apoptosis induced by serum hungry in vitro, Methods Osteoblasts of second or third generation from newly born SD rats ealvaria were divided randomly into the control group, serum hungry group and serum hungry with estrogen group. Cells of each group were incubated for 24 h, 48 h, 72 h, 5 d, 7 d and 14 d, then labeled using TUNEL staining and examined for morphological characteristics of apoptotie cell under light microscopy after incubated for 72 h, The rates of apoptotie cells of each group were examined with flow cytometry. Results The cells of the control group showed normal appears, the serum hungry group had many cells with purple and blue particles in nuclei, but serum hungry with estrogen group had less such cells, The rate of apoptotic cell significantly increased in serum hungry group and decreased in serum hungry with estrogen group compared with the control group examined with flow eytometry （ P 〈0. 05）. Conclusion Estrogen can repress osteoblasts apoptosis of rats induced by serum hungry.