厦门地区大肠癌人群某些遗传性易感因子和抗性因子的研究

Sensitive and resistant genetic factors related to colorectal cancer in patients from Xiamen

目的:比较15个STR基因座基因频率在厦门地区大肠癌患者和正常人群中的分布,推测与大肠癌相关的基因．方法:应用PCR复合扩增结合四色荧光检测方法对血样DNA进行基因型分析,调查了本地区大肠癌患者人群和无关人群的基因频率分布,并根据二者的该15个基因座等位基因频率分布的显著性差异,推测易感连锁和抗性连锁的等位基因．结果:厦门地区大肠癌患者的D5S818(0．5200 vs 0,219 5,X2=36．69,P<0．01;RR=3．8521, P<0．05)、vWA(0．0500 vs 0．2927,X2=53．99, P<0．01;RR=0．1272,P<0．05)和FAG(0．09 vs 0．2439,X2=37．58,P<0．01:RR=0．3066, P<0．05)基因座的等位基因的分布与该地区健康人群有显著性差异,(P<0．01)．B组超微结构改变明显,而C组较B组超微结构有不同程度减轻．结论:D5S818-11附近可能存在大肠癌易感基因;vWA-15、FAG-23附近有可能存在与大肠癌相关的抗性基因．

AIM： To compare the gene frequencies of 15 STR loci between patients with colorectal cancer and healthy people from Xiamen in order to search for the genes that related to the colorectal cancer. METHODS： The genotypes of the sample DNA were analyzed by multiplex polymerase chain reaction （PCR） combined with 4-colored fluorescence-labeled method. All the polymorphic alleles of these 15 STR loci in the unrelated healthy locals and patients with colorectal cancer were investigated. The sensitive or resistant genetic factors were inferred according to the statistical difference in the distribution of allele frequencies. RESULTS： There were statistical differences between the healthy controls and patients with colorectal cancer in allele frequencies of the three loci： D5S818 （0.520 0 vs 0.219 5, X^2= 36.69, P 〈 0.01; RR = 3.852 1, P 〈 0.05）, vWA （0.050 0 vs 0.292 7, X^2 = 53.99, P 〈 0.01; RR = 0.127 2, P 〈 0.05）, and FAG （0.09 vs 0.243 9, X^2= 37.58, P 〈 0.01; RR =0.306 6, P 〈 0.05）. CONCLUSION： It is very possible that there is a sensitive gene for colorectal cancer near the area of D5S818-11 locus, and there are resistant genes for colorectal cancer near the region of vWA-15 and FAG-23 locus.