E-选择素在鼻息肉中的分泌与表达

Secretion and expression of E-selectin in nasal polyps

目的:检测黏附分子E选择素在鼻息肉中的分泌水平与表达部位,借以探讨其与鼻息肉可能的病理机制的相关性。方法:采用酶联免疫吸附测定法检测25例鼻息肉(鼻息肉组)组织匀浆中E选择素水平,以9例鼻中隔手术患者之代偿肥大的下鼻甲后端黏膜作为对照(对照组);同时对上述标本进行苏木精伊红染色及E选择素免疫组织化学染色,观察其表达情况。结果:鼻息肉组大量嗜酸粒细胞浸润。E选择素浓度为(42.58±13.52)μg/L,对照组为(11.35±3.17)μg/L,差异有统计学意义(P<0.01)。E选择素主要表达于血管内皮、腺体上皮、间质和浸润炎症细胞上。鼻息肉组腺体上皮阳性表达率为92.0%,对照组为55.6%,差异有统计学意义(P<0.05)。鼻息肉组在血管内皮上阳性表达率为84.0%,对照组为44.4%;鼻息肉组在间质及浸润炎症细胞阳性表达率为92.0%,对照组为33.3%,差异均有统计学意义。Spearman相关性分析E选择素在血管内皮上的表达和在间质及浸润炎症细胞上的表达呈正相关(r=0.544,P<0.01)。结论:高表达的E选择素可能通过参与嗜酸粒细胞等炎症细胞的浸润、活化、增殖,从而在鼻息肉的形成过程中起重要作用。

Objective： To investigate the significance of E-selectin in nasal polyps. Method：The E-selectin was measured by enzyme linked immunosorbent assay in 25 patients and 9 control cases from polyp tissue and normal inferior turbinates respectively. Meanwhile, the expression of E-selectin in these samples was studied with immunohistochemistry. All these samples were also studied by HE stain and observed under optical microscope. Result： Plentiful eosinophils infiltrated the matrix were observed in nasal polyps group. The level of E-selectin was（42.58 ± 13.52） μg/L, significantly higer than that of controls （ 11. 35 ±3. 17） μg/L, （ P〈0. 001 ）. E-selectin express mainly in the vascular endothelial, the gland cells and the extracellular matrix with infiltrated inflammation cells of nasal polyps. The expressions in the vascular endothelial and the matrix of nasal polyps were significantly higher than those of inferior turbinates （84. 0% and 92. 0%, P = 0. 05,0. 01, respectively）. The statistical analysis showed there was positive correlation between the expression of E-selectin in the vascular endothelial and in the matrix（ r =0. 544, P 〈0.01）. Conclusion：This study demonstrates that the secretion and expression of E-selectin in nasal polyps increase significantly. E-selectin may play a key role involving in＇the occurrence of nasal polyps in the chronic inflammation procedure with inflammation cells to attach to the vascular, to migrate into the matrix as well as to be activated.