用ITS和ISSR分子标记技术鉴别香菇生产用种

Use of ITS and ISSR markers to identify cultivated strains for Lentinula edodes

通过选用香菇生产中存在名称争议或者名称相近或者同一名称但长期在不同地区栽培的12株香菇生产菌株,以及用于种水平对比的豹皮香菇Lentinuslepideus和虎皮香菇Lentinustigrinus的4个菌株,共16个菌株作为供试材料,进行ITS和ISSR遗传分析,并用RAPD技术验证试验结果。结果证明,不同种的ITS长度存在差异,再次证明ITS可以有效区别种之间的菌株;在ISSR的菌株水平分析中,香菇种内材料拥有两个共同的条带,与其他两种菌株的带型图谱有着明显差异,其中5对材料的带型图谱极为相近。RAPD验证结果与上述结果相近。由此可见,结合ITS与ISSR技术是可以用作香菇生产菌株鉴别的,这为ITS和ISSR分子标记技术推广应用于香菇生产菌株的快速准确鉴别提供了技术依据。

16 samples were selected including 12 cultivated strains with similar or confused names for Lentinula edodes, 3 strains for Lentinus tigrinus, and one strain for Lentinus lepideus in this study. These samples were firstly analyzed at the level of species by ITS technique, and then were analyzed by ISSR technique. The sizes of the fragments from 3 species amplified by ITS technique were different, which can be used to identify strains from different species. Two fragments owned to Lentinula edodes strains were obtained and 5 pairs of strains for Lentinula edodes showed the same band patterns each other while strains from different species had different band patterns by ISSR analysis. The results were proved by RAPD analysis. In a conclusion, ITS and ISSR techniques can be used to identify cultivated strains for Lentinula edodes.