益妇宁软胶囊对去卵巢大鼠骨形态和生物力学性能的影响

Effects of Yifuning soft gelatin capsules on pathology and biomechanical indexes of ovariectomied rats

目的:观察益妇宁软胶囊(YFN)对去卵巢大鼠骨形态和生物力学性能的影响。方法:采用未孕产SD雌性大鼠60只,50只行双侧卵巢切除术,随机分为YFN大、中、小剂量组和阳性药对照组、模型对照组,每组10只;另10只为假手术组。术后4周开始给药,给药12周后收集标本。采用病理计量学观察骨组织形态并测量骨小梁密度,采用万能生物材料测试机测定骨生物力学指标。结果:YFN能明显增加骨小梁密度,增加股骨、椎骨最大载荷。结论:YFN能显著改善骨质疏松程度,提高骨生物力学性能。

Objective： To study the effects of Yifuning soft gelatin capsules（YFN） on pathology and biomechanical indexes of ovariectotaled female rats. Method： Sixty female Sprague-Dawley rats without pregnancy and delivery were used,50 of them were ovariectomized and randomly divided into 5 groups： ovariectomy（OVX） ,OVX with diethylstilbestrol tables（DT）, OVX with YFN（high dose,middle dose and low dose）, the others were sham-operated group. The rats began to take the rats drugs in the fifth week after the operation. After 12 weeks of treat ment the rats were killed, and the bones were collected to inspect. The pathology of bone was observed with the HE stain and the area of trabecular was detected. The electronic testing device was used to detect the biomechanical properties. Result： The results indicated that the rat model of postmenopausal osteoperosis（PMO） was a success. Compared with the model group, after application of the drugs, the observation of HE showed different repairing around trabecular. The biomechanical parameters also increased significantly. Conclusion： YFN can effectively prevent the loss of bone in OVX rats and strengthen bone quality, and has a good effect on PMO oostmenopausal osteoporosis.