川芎嗪联用异丙酚对实验性缺血-再灌注损伤肝脏能量代谢的干预

Effect of Ligustrazini and Propofol on Hepatocellular Energy Metabolism in the Reperfusion Injury after Hepatic Ischemia in Rabbits

目的探讨川芎嗪(LGT)、异丙酚(PRO)联合使用对肝缺血-再灌注损伤(HIRI)时肝细胞能量代谢的影响及其机制。方法实验兔40只,随机分为肝缺血-再灌注组(A组)和肝缺血-再灌注+LGT治疗组(B组)、肝缺血-再灌注+PRO治疗组(C组)和肝缺血-再灌注+LGT+PRO治疗组(D组)。在再灌注45min时,分别检测肝组织内三磷酸腺苷(ATP)、二磷酸腺苷(ADP)、一磷酸腺苷(AMP)含量及总腺苷酸量(TAN)、能荷(EC)、丙二醛浓度(MDA)、超氧化物歧化酶活性(SOD)、一氧化氮代谢产物(NO2-/NO3-)水平。结果与A组比较,B、C、D组肝组织内ATP、EC、NO2-/NO3-及SOD活性均明显增高(P<0.05和P<0.01),MDA含量显著减少(P<0.01)。结论LGT联用PRO可通过降低体内氧自由基水平、提高一氧化氮(NO)水平,而改善缺血-再灌注损伤肝脏的能量代谢。

ObJective To study the effect of ligustrazini （LGT） and propofol （PRO） on hepatocellular energy metabolism in the reperfusion injury after hepatic ischemia in rabbits and its mechanism. Methods The rabbits were randomly divided into four groups （n=10 in each）, hepatic ischemia-reperfusion （HIR） group, HIR＋LGT group （B）, HIR＋PRO group （C） and HIR＋ LGT＋PRO group （D）. The contents of adenosine triphosphate （ATP）, adenosine diphosphate （ADP）, adenosine monophosphate （AMP）, total adenylic acid number （TAN）, energy charge （EC）, malondialdehyde （MDA）, superoxide dismutase （SOD） and nitric oxide products （NO2^-/NO3^-） in the liver tissue were measured at 45 minutes after reperfusion. Results The contents of ATP, NO and SOD activity of the liver tissue in B, C, D groups were higher than those in HIR group （P〈0.05 and P〈0.01）, the content of MDA of the liver tissue in B, C, D groups were lower than those in HIR group （P〈0.05 and P〈0.01I. Conclusion LGT＋PRO can improve hepatoceUular energy metabolism in the reperfusion injury after liver ischemia by decreasing oxygen free radical level and raising NO level.