土茯苓对白细胞介素-1诱导的血管细胞粘附分子-1表达的影响

Inhibition of Smilax Glabra Roxb on VCAM-1 Expression Induced by IL-1 of Human Vascular Endothelial Cells

目的探讨土茯苓对白介素-1(IL-1)诱导的血管内皮细胞表面的血管细胞粘附分子-1(VCAM-1)表达升高的保护作用。方法以原代培养的人脐静脉内皮细胞(HUVECs)为研究对象,采用中药血清药理学方法、免疫荧光染色、流式细胞仪等技术,以平均荧光强度代表细胞VCAM-1水平。结果IL-1刺激后可升高HUVECs的平均荧光强度(P<0.001),土茯苓含药血清组的平均荧光强度明显低于空白血清组(P<0.05或P<0.01)。结论土茯苓可抑制HUVECs表达VCAM-1。

Objective To investigate the protective effect of Smilax Glabra Roxb on the expression of vascular cell adhesion molecule-1 （VCAM-1） induced by IL-1 in human vascular endothelial cells （HUVECs）. Methods Take human umbilical vascular endothelial cells as study subject, use immunofluorescence staining, serum pharmacology of the traditional Chinese medicine and flow cytometer. The level of VCAM-1 is represented by the mean fluorescence intensity. Results IL-1 increases the expression of VCAM-1, the expression of VCAM-1 in group of serum containing drugs is lower than control group. Conclusion Smilax Glabra Roxb can inhibit the effect of IL-1 induced increase of VCAM-1 expression in HUVECs.