啤酒废酵母核糖核酸提取过程中去除蛋白质的研究

Removal of protein in RNA extraction of waste brewing yeast

讨论了3种去除核糖核酸(RNA)中蛋白质的方法,通过对蛋白质去除效果及影响RNA损失情况的分析比较,发现添加40U/g绝干酵母中性蛋白酶降解蛋白质的方法为最好,这种方法制得RNA粗品,得率达到5.75%,蛋白质去除了96.6%。

Three methods of removing proteins in RNA were discussed in this paper. The best way was to add 40 U neutral protease per gram dry yeast, and to compare the effects of protein removal to the loss of RNA. The extraction yield of this crude RNA product was 5.75 % while the removal ratio of protein was 96.6 %.