摘要
目的:建立河北道地药材北柴胡的 HPLC-ELSD 指纹图谱分析方法,以提高柴胡药材质量标准。方法:采用 HPLC-ELSD 法测定了道地北柴胡等21个不同来源的柴胡样品。色谱条件:C_(18)柱,乙腈-水为流动相进行梯度洗脱,ELSD 检测器,流速1.0mL·min^(-1),柱温30℃。结果:建立了北柴胡 HPLC-ELSD 指纹图谱共有模式,并对不同产地柴胡药材进行了相似度比较。结论:色谱指纹图谱分析法能简便、快速地鉴别和区分不同来源的柴胡药材,为全面控制柴胡药材的质量提供了依据。
Objective： To establish a fingerprint analysis method for identification of Bupleurum chinense DC. of Hebei province and quality control of Radix Bupleuri. Methods： The HPLC - ELSD fingerprints of Radix Bupleuri from different places were obtained from Waters 1525 instruments. The HPLC separation was performed on a C18 sanalytical column gradient eluted with a mixture consisting of acetonitrile and water at a flow rate of 1.0 mL × min^-1 with ELSD detector. The temperature of column was 30 ℃. Results： The mutual mode of HPLC - ELSD fingerprints were set up, and the similar degrees to the crude drugs collected in different areas were compared. Conclusion： It is simple and quick to verity Bupleurum chinense from different sources with the method, and can be used as a quality control item for Radix Bupleuri.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第2期162-167,共6页
Chinese Journal of Pharmaceutical Analysis
基金
2003年河北省自然科学基金项目(303453)
