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高效液相色谱法测定人血浆中双氯西林和阿莫西林的含量 被引量:3

HPLC determination of dicloxacillin and amoxicillin in human plasma
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摘要 目的:建立人血浆中双氯西林和阿莫西林的测定方法。方法:高效液相色谱法,血浆样品以甲醇除蛋白,色谱柱为岛津Shim-pack VP-ODS C_(18)(150mm×4.6mm,5μm),双氯西林的测定以0.05mol·L^(-1)磷酸盐缓冲液(pH 4.5)-乙腈(68:32),加0.3%三乙胺为流动相,检测波长为225nm,柱温为25℃;阿莫西林的测定以0.033mol·L^(-1)磷酸盐缓冲液(pH7.2)-甲醇(88:12)为流动相,检测波长为229nm,柱温为20℃,流速均为1.0mL·min^(-1),进样量均为20μL。结果:在不同的色谱条件下,血浆样品中双氯西林和阿莫西林无干扰,双氯西林的线性范围为0.2~102.4μg·mL^(-1),r=0.9999,最低检测浓度为0.2μg·mL^(-1);阿莫西林的线性范围为0.25~128.0μg·mL^(-1),r=0.9998,最低检测浓度为0.25μg·mL^(-1)。双氯西林和阿莫西林的平均回收率分别为104.2%,101.6%。结论:这种以相同的样品预处理方法,不同的色谱分离条件分析双氯西林和阿莫西林的方法简便、准确、可靠,结果稳定,可用于阿双西林胶囊或片剂的药代动力学研究和生物利用度研究。 Objective: To establish HPLC methods for determination of dicloxacillin and amoxicillin in human plasma. Methods:Plasma samples were pretreated by direct deproteinization with methanol. The chromatographic separation dicloxacillin and amoxicillin from human plasma were achieved using C18 (150 mm ×4. 6 mm,5 μm)column and a mobile phase, consisting of acetonitrile - phosphate solution - triethylamine solution( 65: 32: 0. 3 ) and methanol - phosphate solution(88: 12) , respectively. They were detected at 225 nm and 229 nm, respectively, and the column temperature was 25 ℃ and 20 ℃ , respectively, and flow rate was 1.0 mL · min^- 1 with the sampling volume 20 μL. Results:In the different chromatographic conditions dicloxacillin and amoxicillin were separated completely without interference. The average recovery of dicloxacillin and amoxicillin was 104.2% and 101.6% ,respectively, and the minimum detection concentration of dicloxacillin and amoxicillin was 0. 2 μg · mL^-1 and 0. 25 μg · mL^-1, respectively. The calibration curves were linear over the concentration range of 0. 2 - 102.4 μg · mL^-1 for dicloxacillin and 0. 25 - 128.0 μg · mL^- 1 for amoxicillin with correlation coefficients greater than 0. 999. Conclusion: Both high -performance liquid chromatographic methods by the same sample pretreatment are simple, accurate, stable and suitable for studying the pharmacokinetics and relative bioavailability of dicloxacillin and amoxicillin capsules or tablets.
出处 《药物分析杂志》 CAS CSCD 北大核心 2006年第2期228-231,共4页 Chinese Journal of Pharmaceutical Analysis
关键词 高效液相色谱法 双氯西林 阿莫西林 HPLC dicloxacillin amoxicillin
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参考文献8

  • 1Usleber E, Lorber M, Straka M,et al. Enzyme immunoassay for the detection of isoxazolyl penicillin antibiotics in milk. Analyst, 1994,119(12) :2765
  • 2Roder BL, Frimodt - Moller N, Espersen F, et al. Dicloxacillin and flucloxacillin: pharmacokinetics, protein binding and serum bactericidal titers in healthy subjects after oral administration. Infection,1995,23(2) :107
  • 3张丹,陈红.RP-HPLC法对双氯西林钠及其有关物质的测定研究[J].中国抗生素杂志,2002,27(10):596-598. 被引量:4
  • 4Charles BG, Foo CC, Gath J. Rapid column liquid chromatographic analysis of flueloxaeillin in plasma on a microparticulate pie - column.J Chromatogr B Biomed Sci Appl, 1994,60 (1) :186
  • 5Hung CT, Lira JKC, Zoest FAR. Optimization of high -performance liquid chromatographic analysis for isoxazolyl penicillins using factorial design. J Chromatogr Biomed Appl, 1988,425:331
  • 6Zhi Yuan, Russlie Henry Q, Cannfax Danid M. Sensitive assay for measuring amoxlcillin in human plasma and middle ear fluid using solid - phase extraction and reversed - phase high - performance liquid chromatography. J Chromatogr B, 1995,674 : 93
  • 7Carlqvist J, Westerlund D. Automated determination of amoxieillin in biological fluids by column switching in ion - pair reversed - phase liquid chromatographic systems with post - column derivatization. J Chromatogr Biomed Appl , 1985,334:285
  • 8谭力,周继红,罗楠,袁倚盛.高效液相色谱法测定人血浆中阿莫西林浓度及药代动力学[J].药学学报,1997,32(7):558-560. 被引量:15

二级参考文献2

  • 1National Committee of Pharmacopoeia. Pharmacopoeia of Peoples Republic of China(中华人民共和国药典)[S](Vol Ⅱ). Beijing: The People's Healthy Publishing House, 1978:62
  • 2United States Pharmacopeial Convention Inc. The United States Pharmaeopoeia ZZ Ⅳ [S]. Philadelphia: The Board of Trustees, 2000: 584

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