川产黄花蒿中桉油精、蒿酮、樟脑、石竹烯、氧化石竹烯含量的气相色谱法测定

GC determination of eucalyptol,artemisia ketone,camphor,caryophyllene and caryophyllene oxide in Artemisia annua L.produced in Sichuan

目的:建立川产黄花蒿中桉油精、蒿酮、樟脑、石竹烯、氧化石竹烯含量的气相色谱测定方法。方法:采用岛津 GC-14B气相色谱仪,FID 检测器,DB-1石英毛细管柱(30m×0.25mm×0.25μm),氮气为载气,以十二烷为内标物进行测定。结果:在该色谱条件下,样品中桉油精、蒿酮、樟脑、石竹烯、氧化石竹烯与内标物十二烷能够较好分离,加样回收率桉油精为97.11%(RSD=3.1%);蒿酮为95.52%(RSD=4.2%);樟脑为95.64%(RSD=4.5%);石竹烯为96.81%(RSD=3.9%);氧化石竹烯为95.89%(RSD=4.6%)。结论:本法灵敏、准确,重复性好,可用于黄花蒿中挥发性成分的质量控制。

Objective： To establish the GC determination of eucalyptol, artemisia ketone, camphor, caryophyllene and caryophyllene oxide in Artemisia annua L. produced in Sichuan. Method ： The internal standard method was employed with dodecane as the internal standard;The GC system consisted of Shimadzu GC - 14B with a flame - i- onization detector, DB - 1 capillary column （30 m × 0. 25 mm × 0. 25 μm）, nitrogen as the carrier gas. Result： Eucalyptol, artemisia ketone, Camphor, caryophyllene, caryophyllene oxide and dodecane have been separated well under the chromatographic condition. The mean recovery of eucalyptol, artemisia ketone, camphor, caryophyllene and caryophyllene oxide were 97. 11% （ RSD = 3.1% ）, 95.52% （ RSD = 4. 2% ）, 95.64% （ RSD = 4. 5% ）, 96. 81% （RSD = 3.9% ） ,95.89% （RSD =4. 6% ） respectively. Conclusion： The method is sensitive, accurate and can be used to control the quality of Artemisia annua L.